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General Information
Symbol
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus
Species
C. noctigama
Name
FlyBase ID
FBal0351548
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-CsChrimson, 20XUAS-CsChrimson-mVenus, UAS-Chrimson, 20XUAS-IVS-CsChrimson.mVenus, CsChrimson, UAS-CsChrimson.mVenus, UAS-CsChrimson::mVenus, UAS-CsChrimson-mVenus, UAS-IVS-CsChrimson.mVenus
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

20xUAS drives expression of CsChrimson fused to Venus. Golgi and ER export motifs have also been added to the coding sequence. FlyBase curator comment: the original published version of FBrf0224260 erroneously stated that this construct contained Chrimson rather than CsChrimson; this has since been corrected in the online published version (for details see also the corrigendum FBrf0226484 and the addendum FBrf0227011).

Allele components
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In larvae expressing Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus in motor neurons (via Scer\GAL4VGlut-OK371), both 470-nm and 617-nm light triggers EJPs, even at short (1- to 2-ms) light-pulse durations and low intensities. In response to light-pulse durations over 2 ms, Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus expression triggers long-lasting barrages of EJPs. Long-wavelength red light (720 nm) also triggers EJPs, though high-intensity light and long light-pulse durations (40–160 ms) are required for robust activation of the NMJ.

In adults expressing Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus in sweet taste receptor cells (via Scer\GAL4Gr64f.9.7), proboscis extension reflexes are robustly elicited at very low 470 nm and 617 nm light intensities. Flies also respond to 720-nm light at an intensity substantially lower than that required in the larvae.

Adults expressing Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus in PNv-1 antennal lobe neurons that innervate the V glomerulus (via Scer\GAL4VT31497) which induces an avoidance response to CO[[2]], reliably avoid quadrants in a circular arena lit by weak red light (617 nm).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Suppressor of
Phenotype Manifest In
Suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (16)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus
Cnoc\ChR1Cs.IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus
Cnoc\ChR1Cs.IVS.20xUAS.Venus
Zzzz\ChR1Cs.IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus
Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus
Name Synonyms
Secondary FlyBase IDs
  • FBal0321310
  • FBal0320696
  • FBal0295146
  • FBal0351218
References (80)