FB2025_01 , released February 20, 2025
Allele: Dmel\Vps8UAS.cLa
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General Information
Symbol
Dmel\Vps8UAS.cLa
Species
D. melanogaster
Name
UAS construct a of Lorincz
FlyBase ID
FBal0351685
Feature type
allele
Associated gene
Dmel\Vps8
Associated Insertion(s)
Carried in Construct
P{UAS-Vps8.L}
Key Links
Transgenic product class
cDNA
(Lőrincz et al., 2019)
wild_type
(Lőrincz et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lőrincz et al., 2019)
wild_type
(Lőrincz et al., 2019)
Mutagen
in vitro construct
(Lőrincz et al., 2019)
Progenitor genotype
Carried in construct
P{UAS-Vps8.L}
Cytology
Description

UASt regulatory sequences drive expression of the Vps8 coding sequence (derived from the AT14809 cDNA).

(Lőrincz et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Vps8
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Garland cells expressing Vps8UAS.cLa under the control of Scer\GAL4pros.PU are enlarged, show enlarged late endosomes (Rab7-positive puncta), but not early endosomes (Rab5-positive puncta).

      Under starvation, larval fat body clones expressing Vps8UAS.cLa under the control of Scer\GAL4Act.PU lack the numerous bright lysosome (Lysotracker- or Lamp-positive) and autolysosome (mCh-Atg8a-positive) puncta normally observed in controls, showing instead 'clouds' of many faint lysosomes and autophagosomes (endogenous Atg8a-positive puncta); this, together with an increase in dual green/red mCh-GFP-Atg8a puncta, indicates a block in autophagic flux. These clones also show enlarged Rab7-positive puncta, which represent both autophagosomes and lysosomes in these cells.

      In larval salivary glands expressing Vps8UAS.cLa under the control of Scer\GAL4fkh.PH, lysosomes (Lamp-positive structures) fail to encircle glue granules and instead show a punctate pattern.

      Adults expressing Vps8UAS.cLa under the combined control of Scer\GAL4ey.PU and Scer\GAL4GMR.PF have darker eyes than age-matched controls, and the pseudopupil is completely absent: instead, the center of the eye appears lighter.

      (Lőrincz et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Not rescued by

      Vps8UAS.cLa/Df(3L)ED211 is not rescued by Vps81

      (Lőrincz et al., 2019)
      Fails to rescue

      Vps8UAS.cLa/Scer\GAL4pros.PU fails to rescue Vps81/Df(3L)ED211

      (Lőrincz et al., 2019)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Vps8UAS.cLa
      Name Synonyms
      UAS construct a of Lorincz
      Secondary FlyBase IDs
        References (1)