FB2025_01 , released February 20, 2025
Allele: Dmel\Acox1CR00998-TG4.1
Open Close
General Information
Symbol
Dmel\Acox1CR00998-TG4.1
Species
D. melanogaster
Name
FlyBase ID
FBal0352035
Feature type
allele
Associated gene
Dmel\Acox1
Associated Insertion(s)
TI{CRIMIC.TG4.1}Acox1CR00998-TG4.1
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Chung et al., 2020)
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{CRIMIC.TG4.1}Acox1CR00998-TG4.1
Cytology
Description

A TI{CRIMIC.TG4.1} DNA cassette has been inserted into CG5009, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: ATGTACATGTAGGTCTGTCGGGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.1} cassette is predicted to be accompanied by a deletion of 5bp of genomic sequence flanking the insertion site.

(Gene Disruption Project members, 2018-)
Allele components
Component
Use(s)
Inserted element
TI{CRIMIC.TG4.1}
Encoded product / tool
GAL4
Also carries
attP
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Mitchell syndrome
      CEA
      (Chung et al., 2020)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  Mitchell syndrome
      is ameliorated by Hsap\ACOX1UAS.cCa
      (Chung et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ACOX1CR00998-TG4.1 homozygous adults exhibit progressive climbing defects when compared to controls.

      In ACOX1CR00998-TG4.1 homozygous adults, the number of axons in wing nerves is decreased, many remaining axons are irregular in shape, and most of the wrapping glia are absent or highly aberrant in size and shape when compared to the controls.

      ACOX1CR00998-TG4.1 homozygous adults exhibit a progressive loss of electroretinogram on- and off transients and a decrease in amplitude when compared to controls. Also ACOX1CR00998-TG4.1 flies raised in the dark exhibit neurodegeneration of the retina.

      (Chung et al., 2020)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The electroretinogram phenotype of ACOX1CR00998-TG4.1 homozygous adults is suppressible by expression of Hsap\ACOX1UAS.cCa under the control of Scer\GAL4ACOX1-CR00998-TG4.1.

      (Chung et al., 2020)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      Acox1CR00998-TG4.1 is partially rescued by Acox1+tCH322-174F04

      (Chung et al., 2020)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      ACOX1CR00998-TG4.1
      Acox1CR00998-TG4.1
      CG5009CR00998-TG4.1
      dACOX1T2A
      (Chung et al., 2020)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)