FlyBase Allele Report: Dmel\Ae2[1]

General Information

Symbol

Dmel\Ae2¹

Species

D. melanogaster

Name

FlyBase ID

FBal0352208

Feature type

allele

Associated gene

Dmel\Ae2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Benitez et al., 2019)

Mutagen

CRISPR/Cas9

(Benitez et al., 2019)

Progenitor genotype

Cytology

Description

A 7 base pair deletion causing a frameshift near the 5' end of the coding region (common to all annotated isoforms) that results in an early stop codon.

(Benitez et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:9,779,872..9,779,878 [+]

Comment:

A 7 base pair deletion causing a frameshift near the 5' end of the coding region (common to all annotated isoforms) that results in an early stop codon.

(Benitez et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | female | recessive

(Benitez et al., 2019)

abnormal size | oogenesis (with Ae2²)

(Benitez et al., 2019)

abnormal size | oogenesis | recessive

(Benitez et al., 2019)

decreased fecundity | female | recessive

(Benitez et al., 2019)

increased cell death | oogenesis

(Benitez et al., 2019)

increased cell death | oogenesis | recessive

(Benitez et al., 2019)

viable

(Benitez et al., 2019)

Phenotype Manifest In

basal stalk

(Benitez et al., 2019)

basal stalk (with Ae2²)

(Benitez et al., 2019)

egg chamber

(Benitez et al., 2019)

egg chamber (with Ae2²)

(Benitez et al., 2019)

ovariole

(Benitez et al., 2019)

ovariole (with Ae2²)

(Benitez et al., 2019)

ovary

(Benitez et al., 2019)

Detailed Description

Statement

Reference

Ae2¹ homozygous females present smaller ovaries, with fewer ovarioles, as compared to heterozygous controls. The ovarioles of Ae2¹ homozygous and Ae2¹/Ae2² transheterozygous females present significantly fewer mid-stage follicles/egg chambers (i.e. between the germarium and late stages) and other defects include enlarged basal stalks, fused egg chambers, apoptotic germ cells, abnormally small follicles and a reduced number of mid-stage follicles.

(Benitez et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ae2¹ has abnormal size | recessive | female | adult stage phenotype, suppressible by +/Dp(3;2)GV-CH321-06I06

(Benitez et al., 2019)

Ae2¹ has decreased fecundity | recessive | female phenotype, suppressible by +/Dp(3;2)GV-CH321-06I06

(Benitez et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ae2¹ has ovary phenotype, suppressible by +/Dp(3;2)GV-CH321-06I06

(Benitez et al., 2019)

Ae2¹ has ovariole phenotype, suppressible by +/Dp(3;2)GV-CH321-06I06

(Benitez et al., 2019)

Ae2¹ has egg chamber phenotype, suppressible by +/Dp(3;2)GV-CH321-06I06

(Benitez et al., 2019)

Ae2¹ has ovary phenotype, suppressible by Nhe2[+]/Nhe2^null

(Benitez et al., 2019)

Ae2¹ has ovariole phenotype, suppressible by Nhe2[+]/Nhe2^null

(Benitez et al., 2019)

Other

Statement

Reference

Ae2[+]/Ae2¹, Nhe2^UAS.cGa, Scer\GAL4^109-30 has egg chamber phenotype

(Benitez et al., 2019)

Ae2[+]/Ae2¹, Nhe2^UAS.cGa, Scer\GAL4^109-30 has ovariole phenotype

(Benitez et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Expressing Nhe2^UAS.cGa under the control of Scer\GAL4^109-30 in a Ae2¹ heterozygous or homozygous background leads to significantly fewer mid-stage follicles/egg chambers (i.e. between the germarium and late stages), as compared to controls.

(Benitez et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ae2¹

ae2¹

(Benitez et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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