FB2025_01 , released February 20, 2025
Allele: Dmel\notWKO.P5-C6
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General Information
Symbol
Dmel\notWKO.P5-C6
Species
D. melanogaster
Name
FlyBase ID
FBal0352421
Feature type
allele
Associated gene
Dmel\not
Associated Insertion(s)
Carried in Construct
M{WKO.P5-C6}
Key Links
Transgenic product class
sgRNA
(Meltzer, 2019-)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Meltzer, 2019-)
Mutagen
in vitro construct
(Meltzer, 2019-)
Progenitor genotype
Carried in construct
M{WKO.P5-C6}
Cytology
Description

CRISPR transgene designed to result in an indel (small insertion or deletion mutation) in a target gene when combined with a source of Cas9. Constitutively expresses two sgRNAs, both of which target sequence within the coding region of not, immediately downstream of the translation initiation site.

(Meltzer, 2019-)
Allele components
Component
Use(s)
Encoded product / tool
sgRNA-WKO.P5-C6.1
sgRNA-WKO.P5-C6.2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CRISPR?Cas9 mutagenesis of not by the presence of notWKO.P5-C6 and expressing Spyo\Cas9UAS.P2 under the control of either Scer\GAL4slbo.2.6 or Scer\GAL4c306 induces border cell migration defects.

      (Badmos et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      notWKO.P5-C6
      Name Synonyms
      Secondary FlyBase IDs
        References (2)