FlyBase Allele Report: Dmel\Eaat1[hypo]

General Information

Symbol

Dmel\Eaat1^hypo

Species

D. melanogaster

Name

FlyBase ID

FBal0353067

Feature type

allele

Associated gene

Dmel\Eaat1

Associated Insertion(s)

roo{}Eaat1^hypo

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Peng et al., 2019)

Mutagen

Progenitor genotype

Associated Insertion(s)

roo{}Eaat1^hypo

Cytology

Description

428bp insertion of the LTR of the roo element in intron 10 of Eaat1.

(Peng et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:9,340,681..9,340,682

(Peng et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of episodic ataxia type 6

CEA

(Peng et al., 2019)

CEA with Eaat1^SM2

(Peng et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of episodic ataxia type 6

is ameliorated by Hsap\SOD1^UAS.cWa

(Peng et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage (with Eaat1^SM2)

(Peng et al., 2019)

abnormal locomotor behavior | larval stage

(Peng et al., 2019)

abnormal locomotor behavior | larval stage (with Eaat1^SM2)

(Peng et al., 2019)

abnormal neuroanatomy | larval stage

(Peng et al., 2019)

abnormal neuroanatomy | larval stage (with Eaat1^SM2)

(Peng et al., 2019)

abnormal neurophysiology | larval stage

(Peng et al., 2019)

abnormal neurophysiology | larval stage (with Eaat1^SM2)

(Peng et al., 2019)

abnormal size | larval stage (with Eaat1^SM2)

(Peng et al., 2019)

lethal - all die before end of larval stage (with Eaat1^SM2)

(Peng et al., 2019)

lethal - all die before end of pupal stage

(Peng et al., 2019)

majority die during larval stage

(Peng et al., 2019)

some die during first instar larval stage (with Eaat1^SM2)

(Peng et al., 2019)

some die during pupal stage

(Peng et al., 2019)

some die during second instar larval stage (with Eaat1^SM2)

(Peng et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Eaat1^hypo homozygous larvae have severely impaired locomotion and their neuromuscular junctions exhibit morphological and functional defects compared to controls: there are more boutons per muscle but boutons (type Ib) are smaller and there are no changes in the number of active zones per muscle; there are significant increases in EJP amplitude, quantal content and burst duration, but no change in mEJP amplitude; there are no changes in muscle area. Eaat1^hypo homozygous larvae do not show any obvious VNC structural defects, exhibiting similar numbers of neurons and astrocytes to controls.

Eaat1^hypo/Eaat1^SM2 transheterozygous larvae have severely impaired locomotion and their neuromuscular junctions exhibit morphological and functional defects compared to controls, namely a significant increase in burst duration and a decrease in burst frequency, significantly more but smaller boutons (including satellite boutons) and smaller muscles.

(Peng et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible | partially by Scer\GAL4^repo/Hsap\SLC1A2^UAS.EGFP

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^repo/Hsap\SLC1A2^UAS.EGFP

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, suppressible by Scer\GAL4^repo/Hsap\SLC1A2^UAS.EGFP

(Peng et al., 2019)

Eaat1^hypo has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^repo/Hsap\SLC1A2^UAS.EGFP

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^C57

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, suppressible | partially by Hsap\SOD1^UAS.cWa/Scer\GAL4^C57

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^C57

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut1-OK371

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^Toll-6-D42

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible | partially by Scer\GAL4^repo/VGlut1^GD834

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible | partially by GluRIID[+]/GluRIID^e01443

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible | partially by Scer\GAL4^VGlut1-OK371/VGlut1^GD834

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, suppressible | partially by Scer\GAL4^VGlut1-OK371/VGlut1^GD834

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by bsk²/bsk[+]

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by kay¹/kay[+]

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^Toll-6-D42/kay^Fbz.UAS

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, suppressible | partially by Scer\GAL4^Toll-6-D42/kay^Fbz.UAS

(Peng et al., 2019)

NOT suppressed by

Statement

Reference

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, non-suppressible by GluClα^glc1/GluClalpha[+]

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, non-suppressible by GluRIID[+]/GluRIID^e01443

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, non-suppressible by GluClα^glc1/GluClalpha[+]

(Peng et al., 2019)

Eaat1^hypo has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Jra^IA109/Jra[+]

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, non-suppressible by Scer\GAL4^Toll-6-D42/kay^Fbz.UAS

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, non-suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut1-OK371

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, non-suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut1-OK371

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, non-suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^Gad1.3.098

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, non-suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^Gad1.3.098

(Peng et al., 2019)

Eaat1^hypo has abnormal neurophysiology | larval stage phenotype, non-suppressible by Scer\GAL4^NP2225/Hsap\SOD1^UAS.cWa

(Peng et al., 2019)

Eaat1^hypo has abnormal locomotor behavior | larval stage phenotype, non-suppressible by Scer\GAL4^NP2225/Hsap\SOD1^UAS.cWa

(Peng et al., 2019)

Other

Statement

Reference

Eaat1^hypo, Hsap\SLC1A2^UAS.EGFP, Scer\GAL4^repo has partially lethal - majority live phenotype

(Peng et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Scer\GAL4^repo/VGlut1^GD834

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by GluRIID[+]/GluRIID^e01443

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Scer\GAL4^VGlut1-OK371/VGlut1^GD834

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by bsk²/bsk[+]

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by bsk²/bsk[+]

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by kay¹/kay[+]

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by kay¹/kay[+]

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Scer\GAL4^Toll-6-D42/kay^Fbz.UAS

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by Scer\GAL4^Toll-6-D42/kay^Fbz.UAS

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Scer\GAL4^repo/Hsap\SLC1A2^UAS.EGFP

(Peng et al., 2019)

Eaat1^hypo has type I bouton | larval stage phenotype, suppressible by Scer\GAL4^repo/Hsap\SLC1A2^UAS.EGFP

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^ChAT.7.4

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^C57

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^C57

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^nSyb.PS

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut1-OK371

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^VGlut1-OK371

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^Toll-6-D42

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^Toll-6-D42

(Peng et al., 2019)

NOT suppressed by

Statement

Reference

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by GluClα^glc1/GluClalpha[+]

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Jra^IA109/Jra[+]

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Scer\GAL4^NP2225/Hsap\SOD1^UAS.cWa

(Peng et al., 2019)

Eaat1^hypo has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Jra^IA109/Jra[+]

(Peng et al., 2019)

Eaat1^hypo has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Hsap\SOD1^UAS.cWa/Scer\GAL4^Gad1.3.098

(Peng et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Eaat1^SM2/Eaat1^hypo is rescued by Scer\GAL4^repo/Eaat1^UAS.Venus

(Peng et al., 2019)

Eaat1^hypo is rescued by Scer\GAL4^repo/Eaat1^UAS.Venus

(Peng et al., 2019)

Partially rescued by

Eaat1^hypo is partially rescued by Eaat1^UAS.Venus/Scer\GAL4^alrm.PD

(Peng et al., 2019)

Eaat1^hypo is partially rescued by Scer\GAL4^nSyb.PS/Eaat1^UAS.Venus

(Peng et al., 2019)

Eaat1^hypo is partially rescued by Scer\GAL4^tsh-Gal4-1/Eaat1^UAS.Venus

(Peng et al., 2019)

Comments

Constitutive expression of Eaat1^UAS.Venus under the control of Scer\GAL4^repo rescues the locomotor defects of Eaat1^hypo homozygous larvae; the same rescue is obtained by conditional expression only during L1-L3 , L2-L3, L3, or mL3 (temporal regulation of expression is under the control of Gal80[ts] and temperature shift from 18[o]C to 29[o]C).

(Peng et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Eaat1^hypo

(Yao, 2019.11.13)

eaat1^hypo

(Peng et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (2)

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