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General Information
Symbol
Dmel\Eaat1hypo
Species
D. melanogaster
Name
FlyBase ID
FBal0353067
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    428bp insertion of the LTR of the roo element in intron 10 of Eaat1.

    Insertion components
    roo{}Eaat1hypo
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Eaat1hypo homozygous larvae have severely impaired locomotion and their neuromuscular junctions exhibit morphological and functional defects compared to controls: there are more boutons per muscle but boutons (type Ib) are smaller and there are no changes in the number of active zones per muscle; there are significant increases in EJP amplitude, quantal content and burst duration, but no change in mEJP amplitude; there are no changes in muscle area. Eaat1hypo homozygous larvae do not show any obvious VNC structural defects, exhibiting similar numbers of neurons and astrocytes to controls.

    Eaat1hypo/Eaat1SM2 transheterozygous larvae have severely impaired locomotion and their neuromuscular junctions exhibit morphological and functional defects compared to controls, namely a significant increase in burst duration and a decrease in burst frequency, significantly more but smaller boutons (including satellite boutons) and smaller muscles.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Statement
    Reference

    Eaat1hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by bsk2/bsk[+]

    Eaat1hypo has abnormal neuroanatomy | larval stage phenotype, suppressible by kay1/kay[+]

    NOT suppressed by
    Statement
    Reference
    Other
    Phenotype Manifest In
    Suppressed by
    Statement
    Reference

    Eaat1hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by bsk2/bsk[+]

    Eaat1hypo has NMJ bouton | increased number | larval stage phenotype, suppressible by kay1/kay[+]

    NOT suppressed by
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Constitutive expression of Eaat1UAS.Venus under the control of Scer\GAL4repo rescues the locomotor defects of Eaat1hypo homozygous larvae; the same rescue is obtained by conditional expression only during L1-L3 , L2-L3, L3, or mL3 (temporal regulation of expression is under the control of Gal80[ts] and temperature shift from 18[o]C to 29[o]C).

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    Eaat1hypo
    Name Synonyms
    Secondary FlyBase IDs
      References (2)