FB2021_02, released April 13, 2021

Allele: Dmel\RafA572E.UAS.Tag:FLAG

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General Information
Symbol
Dmel\RafA572E.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0353579
Feature type
allele
Associated gene
Dmel\Raf
Associated Insertion(s)
Carried in Construct
P{UAS-Raf.A572E.FLAG}
Key Links
Genomic Maps
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Pfeifle et al., 2019)
in vitro construct - coding region fusion
(Pfeifle et al., 2019)
in vitro construct - site directed mutagenesis
(Pfeifle et al., 2019)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point_mutation
X:2,342,926..2,342,926 [+]
Nucleotide change:

C2342926A

Amino acid change:

A572E | Raf-PA; A572E | Raf-PE

Reported amino acid change:

A572E

Comment:

Analogous V600E mutation in human BRAF implicated in cancer, multiple, BRAF-related; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Pfeifle et al., 2019)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Raf.A572E.FLAG}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of Raf cDNA with a A572E mutation (equivalent to the cancer-related Hsap\BRAF V600E mutation in humans), tagged at the N-terminal with Tag:FLAG.

      (Pfeifle et al., 2019)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Raf
      Tagged with
      Tag:FLAG
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Pfeifle et al., 2019)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Pfeifle et al., 2019)
      BRAF:p.Val600Glu
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: BRAF_13961
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RafA572E.UAS.Tag:FLAG
      dRafA572E
      (Pfeifle et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)