FlyBase Allele Report: Avic\GFP[EGFP.UASp.Lifeact]

General Information

Symbol

Avic\GFP^{EGFP.UASp.Lifeact}

Species

A. victoria

Name

FlyBase ID

FBal0355540

Feature type

allele

Associated gene

Avic\GFP

Associated Insertion(s)

Carried in Construct

P{UASp-Lifeact.mEGFP}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Spracklen et al., 2014)

Progenitor genotype

Carried in construct

P{UASp-Lifeact.mEGFP}

Cytology

Description

UASp regulatory sequences drive expression of EGFP tagged with Lifeact.

(Spracklen et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

EGFP

green fluorescent protein

Tagged with

Lifeact

specific binding tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile, with Scer\GAL4^c355

(Spracklen et al., 2014)

female fertile, with Scer\GAL4^{VP16.nanos.UTR}

(Spracklen et al., 2014)

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

female sterile, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

Phenotype Manifest In

egg chamber | oogenesis stage S9, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

egg chamber | oogenesis stage S9, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

egg chamber | oogenesis stage S10B, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

egg chamber | oogenesis stage S10B, with Scer\GAL4^{VP16.nanos.UTR}

(Spracklen et al., 2014)

egg chamber | oogenesis stage S10B, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S9, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S9, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S10B, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S10B, with Scer\GAL4^{VP16.nanos.UTR}

(Spracklen et al., 2014)

filamentous actin | oogenesis stage S10B, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

nurse cell, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

nurse cell, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

oocyte, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

ring canal, with Scer\GAL4^{VP16.mat.αTub67C}

(Spracklen et al., 2014)

ring canal, with Scer\GAL4^VP16.osk

(Spracklen et al., 2014)

Detailed Description

Statement

Reference

Expression of Scer\ABP140^{Scer\UAS.P\T.T:Avic\GFP-EGFP} in the somatic follicle cells under the control of Scer\GAL4^c355 has little or no effect on female fertility, and no apparent defects in F-actin are seen at stage 9 or 10B of follicle development. Similar results are seen when Scer\ABP140^{Scer\UAS.P\T.T:Avic\GFP-EGFP} is expressed using the weak germline driver Scer\GAL4^{nos.UTR.T:Hsim\VP16}, except that mild defects in cortical actin integrity and actin bundle organisation are observed at stage 10B. Conversely strong germline expression under the control of either Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} or Scer\GAL4^{osk.T:Hsim\VP16} results in female sterility. At stage 9 of follicle development, severe defects in F-actin and follicle morphology are seen, including the loss of cortical actin integrity, which results in aggregates of ring canals and multi-nucleate nurse cells, altered nurse cell and oocyte shape and the formation of phalloidin and Scer\ABP140^{Scer\UAS.P\T.T:Avic\GFP-EGFP} threads in the germinal vesicle. At stage 10B cortical actin is compromised, as multinucleate nurse cells are always observed. At 25[o]C, often all 15 nurse cell nuclei are contained within one or two "cells". Aberrant F-actin structures are also observed around the germinal vesicle.

(Spracklen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Avic\GFP^{EGFP.UASp.Lifeact}

Scer\ABP140^{Scer\UAS.P\T.T:Avic\GFP-EGFP}

Scer\ABP140^UASp.EGFP

Name Synonyms

Secondary FlyBase IDs

FBal0355336
FBal0298815

References (2)

Report Sections

Open Close