FlyBase Allele Report: Dmel\polo[T182D.UASp.RR.GFP]

FB2025_05 , released December 11, 2025

FB2025_05 , released December 11, 2025

Allele: Dmel\polo^{T182D.UASp.RR.GFP}

General Information

Symbol

Dmel\polo^{T182D.UASp.RR.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0356543

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UASp-polo.T182D.RR.GFP}

Key Links

Transgenic product class

missense_variant

(Kachaner et al., 2017)

polypeptide_post_translational_processing_variant

(Kachaner et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kachaner et al., 2017)

polypeptide_post_translational_processing_variant

(Kachaner et al., 2017)

Mutagen

in vitro construct

(Kachaner et al., 2017)

Progenitor genotype

Carried in construct

P{UASp-polo.T182D.RR.GFP}

Cytology

Description

UASp regulatory sequences drive expression of polo that carries a T182D amino acid replacement (phosphomimetic mutation at the activation loop phosphorylation site in the kinase domain). The coding sequence is tagged at the C-terminal end with GFP. Synonymous codon replacements have been introduced into the transgene, so that it is insensitive to RNAi mediated by P{GD7563}.

(Kachaner et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Tagged with

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Gallaud et al., 2022)

Phenotype Manifest In

larval neuroblast | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Gallaud et al., 2022)

Detailed Description

Statement

Reference

The expression of polo^{T182A.UASp.RNAi-res.GFP} under the control of Scer\GAL4^insc-Mz1407 induces increased mitosis time in third instar larval neuroblasts.

(Gallaud et al., 2022)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^insc-Mz1407, polo^{T182D.UASp.RR.GFP} has decreased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by mad2^G6595

(Gallaud et al., 2022)

NOT suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, polo^{T182D.UASp.RR.GFP} has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by mad2^G6595

(Gallaud et al., 2022)

NOT Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a non-enhancer of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of increased cell number | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of neoplasia | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of abnormal mitotic cell cycle | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Scer\GAL4^wor.PA/polo^{T182D.UASp.RR.GFP} is a suppressor | partially of decreased occurrence of cell division | third instar larval stage phenotype of aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor | partially of decreased occurrence of cell division | third instar larval stage phenotype of mad2^G6595, polo^S132408/polo^S025604

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

mad2^G6595, Scer\GAL4^insc-Mz1407, polo^{T182D.UASp.RR.GFP} is a suppressor of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a non-suppressor of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^insc-Mz1407, polo^{T182D.UASp.RR.GFP} has larval neuroblast | third instar larval stage phenotype, non-enhanceable by mad2^G6595

(Gallaud et al., 2022)

NOT suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, polo^{T182D.UASp.RR.GFP} has larval neuroblast | third instar larval stage phenotype, non-suppressible by mad2^G6595

(Gallaud et al., 2022)

NOT Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a non-enhancer of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

mad2^G6595, Scer\GAL4^insc-Mz1407, polo^{T182D.UASp.RR.GFP} is a suppressor of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of larval neuroblast | increased number | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor of larval brain | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Scer\GAL4^wor.PA/polo^{T182D.UASp.RR.GFP} is a suppressor | partially of larval neuroblast | third instar larval stage phenotype of aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a suppressor | partially of larval neuroblast | third instar larval stage phenotype of mad2^G6595, polo^S132408/polo^S025604

(Gallaud et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} is a non-suppressor of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP} partially rescues polo^S132408/polo^S025604

(Gallaud et al., 2022)

Fails to rescue

Scer\GAL4^Act5C.PI/polo^{T182D.UASp.RR.GFP} fails to rescue polo^S132408/polo^S025604

(Gallaud et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

polo^{T182D.UASp.RNAi-res.GFP}

polo^{T182D.UASp.RR.GFP}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections