FB2025_05 , released December 11, 2025
Allele: Dmel\poloK54M.UASp.RR.GFP
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General Information
Symbol
Dmel\poloK54M.UASp.RR.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0356545
Feature type
allele
Associated gene
Dmel\polo
Associated Insertion(s)
Carried in Construct
P{UASp-polo.K54M.RR.GFP}
Key Links
Transgenic product class
missense_variant
(Kachaner et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kachaner et al., 2017)
Mutagen
in vitro construct
(Kachaner et al., 2017)
Progenitor genotype
Carried in construct
P{UASp-polo.K54M.RR.GFP}
Cytology
Description

UASp regulatory sequences drive expression of polo that carries a K54M amino acid replacement, resulting in a kinase-dead form of the protein. The coding sequence is tagged at the C-terminal end with GFP. Synonymous codon replacements have been introduced into the transgene, so that it is insensitive to RNAi mediated by P{GD7563}.

(Kachaner et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
polo
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of poloK54M.UASp.RNAi-res.GFP under the control of Scer\GAL4insc-Mz1407 induces increased mitosis time in third instar larval neuroblasts.

      (Gallaud et al., 2022)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      poloK54M.UASp.RNAi-res.GFP
      poloK54M.UASp.RR.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (4)