FlyBase Allele Report: Dmel\rl[R80S.D334N.UAS]

General Information

Symbol

Dmel\rl^{R80S.D334N.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0358599

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{UAS-rl.R80S.D334N}

Key Links

Transgenic product class

missense_variant

(Kushnir et al., 2020)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kushnir et al., 2020)

Mutagen

in vitro construct

(Kushnir et al., 2020)

Progenitor genotype

Carried in construct

P{UAS-rl.R80S.D334N}

Cytology

Description

UASt regulates expression of rl containing a serine instead of an arginine at position amino acid 80 as well as the "sevenmaker" gain-of-function mutation (D334N). The R68S mutation was initially identified in the yeast ERK, Mpk1, and renders Mpk1 intrinsically active; mutations at the equivalent residue of human ERK1 (R84) and ERK2 (R65) have been found in human tumors. The equivalent mutation to D334N is found in cancer patients (D321N in human ERK2).

(Kushnir et al., 2020)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with scrib¹

(Kushnir et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Phenotype Manifest In

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Detailed Description

Statement

Reference

Expressing rl^{R80S.D334N.UAS} under the control of Scer\GAL4^Bx-MS1096 causes dramatic wing vein hypertrophy, with formation of extra vein material, enhanced vein branching, and darker wing pigmentation.

(Kushnir et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of visible | adult stage phenotype of Raf^DN.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of abnormal size | adult stage phenotype of Raf^DN.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of abnormal size | adult stage phenotype of Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor | partially of abnormal size | adult stage phenotype of Dsor1^GD9123, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor | partially of visible | adult stage phenotype of Dsor1^GD9123, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of visible | adult stage phenotype of Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Other

Statement

Reference

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{R80S.D334N.UAS} has partially lethal - majority live phenotype

(Kushnir et al., 2020)

Scer\GAL4^Act5C.PI, rl^{R80S.D334N.UAS}, scrib¹ has abnormal size | somatic clone | larval stage phenotype

(Kushnir et al., 2020)

Scer\GAL4^Act5C.PI, rl^{R80S.D334N.UAS}, scrib¹ has neoplasia | somatic clone | adult stage phenotype

(Kushnir et al., 2020)

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{R80S.D334N.UAS} has visible | adult stage phenotype

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has visible | adult stage phenotype

(Kushnir et al., 2020)

Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has visible | adult stage phenotype

(Kushnir et al., 2020)

Phenotype Manifest In

Suppressor of

Statement

Reference

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Raf^DN.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of wing vein phenotype of Raf^DN.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor | partially of wing phenotype of Dsor1^GD9123, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

rl^{R80S.D334N.UAS}, Scer\GAL4^Bx-MS1096 is a suppressor of wing vein phenotype of Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096

(Kushnir et al., 2020)

Other

Statement

Reference

Dsor1^GD9123, Scer\GAL4^ey.PH, rl^{R80S.D334N.UAS} has eye phenotype

(Kushnir et al., 2020)

Scer\GAL4^Act5C.PI, rl^{R80S.D334N.UAS}, scrib¹ has adult abdomen phenotype

(Kushnir et al., 2020)

Scer\GAL4^Act5C.PI, rl^{R80S.D334N.UAS}, scrib¹ has antennal disc phenotype

(Kushnir et al., 2020)

Raf^DN.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has wing vein phenotype

(Kushnir et al., 2020)

Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has wing phenotype

(Kushnir et al., 2020)

Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has wing vein | ectopic phenotype

(Kushnir et al., 2020)

Ras85D^N17.UAS, Scer\GAL4^Bx-MS1096, rl^{R80S.D334N.UAS} has wing vein phenotype

(Kushnir et al., 2020)

Scer\GAL4^Act5C.PI, rl^{R80S.D334N.UAS}, scrib¹ has eye disc | somatic clone phenotype

(Kushnir et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

The vestigial wing phenotype induced by the expression of Dsor1^GD9123 under the control of Scer\GAL4^Bx-MS1096 is considerably suppressed by the co-expression of rl^{R80S.D334N.UAS}.

The pupal lethality of individuals expressing Dsor1^GD9123 under the control of Scer\GAL4^ey.PH is strongly rescued by the co-expression of rl^{R80S.D334N.UAS} (majority live), although the adults show a (mostly mild) small eye phenotype.

The wing defects induced by the expression of Ras85D^N17.UAS under the control of Scer\GAL4^Bx-MS1096 (smaller wing and loss of L3-5 vein material are fully suppressed by the co-expression of rl^{R80S.D334N.UAS}, even leading to extra vein material, with darker pigmentation).

scrib¹ homozygous eye disc clones that also express rl^{R80S.D334N.UAS} under the control of Scer\GAL4^Act5C.PI are larger than clones only homozygous for scrib¹. Double mutant antennal disc clones are readily detected, unlike scrib¹ single mutant clones. Likewise, Individuals bearing double mutant clones show high abdominal accumulation of clonal cells, which is rare in the scrib¹ single mutant clone condition.

(Kushnir et al., 2020)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rl^{R80S.D334N.UAS}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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