FlyBase Allele Report: Dmel\Xrcc2[CC]

General Information

Symbol

Dmel\Xrcc2^CC

Species

D. melanogaster

Name

FlyBase ID

FBal0359105

Feature type

allele

Associated gene

Dmel\Xrcc2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Bayer et al., 2020)

Mutagen

CRISPR/Cas9

(Bayer et al., 2020)

Progenitor genotype

Cytology

Description

Deletion in Xrcc2; coordinates X:18500252..18501142 , release 6 genome.

(Nagel, 2020.5.15)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:18,500,252..18,501,142 [+]

Comment:

The deletion starts downstream of the AUG and removes most of Xrcc2.

(Nagel, 2020.5.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair | third instar larval stage

(Bayer et al., 2020)

fertile | female

(Bayer et al., 2020)

partially lethal - majority die | conditional

(Bayer et al., 2020)

radiation sensitive

(Bayer et al., 2020)

viable

(Bayer et al., 2020)

Phenotype Manifest In

larval neuroblast | third instar larval stage

(Bayer et al., 2020)

nuclear chromosome | third instar larval stage

(Bayer et al., 2020)

wing disc | third instar larval stage

(Bayer et al., 2020)

X chromosome | adult stage

(Bayer et al., 2020)

Detailed Description

Statement

Reference

Xrcc2^CC homozygous adults don't exhibit any apparent phenotypes and females lay eggs of normal appearance with respect to dorso-ventral patterning when compared to controls.

Xrcc2^CC homozygous female flies exhibit a mild increase in the frequency of X-chromosome nondisjunction while their ability to generate recombinant chromosomes is only slightly compromised when compared to controls.

Xrcc2^CC homozygotes exhibit a high sensitivity to the DNA damaging agents (complete or near complete lethality upon Methyl methanesulfonate or Hydroxyurea treatment, and a decrease in survival in response to Camptothecin or ionizing radiation exposure) when compared to controls.

Xrcc2^CC homozygous third instar larvae exhibit an increase in chromosomal aberrations in larval neuroblasts compared to controls. Also after ionizing radiation exposure the chromosomal defects and the DNA damage response (phosphorylated histone variant H2AX staining) persist much longer although the damage to chromosomes is similar compared to controls.

(Bayer et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Xrcc2^CC/Xrcc2^CC is an enhancer of dorsal appendage phenotype of spn-B²/spn-B^BU

(Bayer et al., 2020)

NOT Enhancer of

Statement

Reference

Xrcc2^CC/Xrcc2^CC is a non-enhancer of dorsal appendage phenotype of spn-A^093A/spn-A¹

(Bayer et al., 2020)

NOT Suppressor of

Statement

Reference

Xrcc2^CC/Xrcc2^CC is a non-suppressor of dorsal appendage phenotype of spn-A^093A/spn-A¹

(Bayer et al., 2020)

Other

Statement

Reference

Xrcc2^CC, spn-A^093A has dorsal appendage phenotype

(Bayer et al., 2020)

Xrcc2^CC, spn-A¹ has dorsal appendage phenotype

(Bayer et al., 2020)

Xrcc2^CC, spn-B² has dorsal appendage phenotype

(Bayer et al., 2020)

Xrcc2^CC, spn-B^BU has dorsal appendage phenotype

(Bayer et al., 2020)

CycG^HR7, Xrcc2^CC has dorsal appendage phenotype

(Bayer et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xrcc2^CC homozygous females in the heterozygous background of CycG^HR7, spn-A^093A, spn-A¹, spn-B² or spn-B^BU lay eggs with defective dorsal appendages (fused, narrowly spaced, branched or shortened) when compared to controls.

(Bayer et al., 2020)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Xrcc2^CC is rescued by Xrcc2^+tBa

(Bayer et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93860

w^* Xrcc2^CC

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Xrcc2^CC

(Nagel, 2020.5.15)

xrcc2^CC

(Bayer et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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