TrpA1 sequence has been inserted into the attP site present in TI{TI}TrpA1KO, reconstituting a wild-type TrpA1 transcription unit in which a TI{2A-GAL4} sequence cassette has been inserted immediately before the endogenous stop codon in exon 18; the presence of the 2A sequence should allow independent translation of the GAL4 driver. A single attR site is present upstream of the introduced TrpA1 sequence, and a single loxP site is present downstream of the introduced TrpA1 sequence.