TrpA1 sequence has been inserted into the attP site present in TI{TI}TrpA1^KO, reconstituting a modified TrpA1 transcription unit that expresses only a single intact isoform; expresses the 'E' isoform (isoform nomenclature of FBrf0211878 and FBrf0217493, corresponds to TrpA1-PK of the FlyBase FB2018_05 release). This has been achieved by introducing a stop codon into exon 3, adding an A nucleotide at the end of exon 12 and adding an A nucleotide at the end of exon 13. In addition, a TI{2A-lexA::UnkAD} sequence cassette has been inserted immediately before the endogenous stop codon in exon 18; the presence of the 2A sequence should allow independent translation of the lexA::UnkAD driver in the expression pattern of the 'E' isoform. A single attR site is present upstream of the introduced TrpA1 sequence, and a single loxP site is present downstream of the introduced TrpA1 sequence.