FB2025_01 , released February 20, 2025
Allele: Dmel\Ras85DV12.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Ras85DV12.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0360566
Feature type
allele
Associated gene
Dmel\Ras85D
Associated Insertion(s)
Carried in Construct
P{UAS-Ras85D.V12.FLAG}
Key Links
Transgenic product class
missense_variant
(Vega-Cuesta et al., 2020)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Vega-Cuesta et al., 2020)
Mutagen
in vitro construct
(Vega-Cuesta et al., 2020)
Progenitor genotype
Carried in construct
P{UAS-Ras85D.V12.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of the Ras85D coding sequence (derived from the RE53955 cDNA clone), mutated to carry the G12V activating mutation. The coding sequence is tagged at the N-terminal end with three copies of Tag:FLAG.

(Vega-Cuesta et al., 2020)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ras85D
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Ras85DV12.UAS.Tag:FLAG under the control of Scer\GAL4salm.EPv leads to wing folding.

      (Vega-Cuesta et al., 2020)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      NOT suppressed by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ras85DV12.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)