FlyBase Allele Report: Dmel\Tgs1[15-11]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\Tgs1^15-11

General Information

Symbol

Dmel\Tgs1^15-11

Species

D. melanogaster

Name

FlyBase ID

FBal0364095

Feature type

allele

Associated gene

Associated Insertion(s)

TI{TI}Tgs1^15-11

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

gene targeting by homologous recombination

(Cheng et al., 2020)

phiC31 integrase

(Cheng et al., 2020)

Progenitor genotype

Associated Insertion(s)

TI{TI}Tgs1^15-11

Cytology

Description

Four alterations in the dicistronic transcription unit that encodes the moi and Tgs1 open reading frames. The alterations are: 1. a DNA sequence encoding 3 copies of the Tag:HA tag has been inserted downstream of the moi translation start codon and in-frame with the moi coding region, 2. an AsisI restriction site has been introduced into the first intron of moi, 3. a GAA to GTA codon change (E30V amino acid substitution) has been inserted into the moi open reading frame, 4. an attR site is present downstream of the Tgs1 3'UTR.

(Cheng et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

moi^CB02140/Tgs1^15-11 testes exhibit meiotic arrest with scattered PH3-positive cells.

(Cheng et al., 2020)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has male sterile phenotype, suppressible by Tgs1^+t4

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has male sterile phenotype, suppressible by Tgs1^t4.moi-G45R

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has male sterile phenotype, suppressible by Tgs1^GFP

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has male sterile phenotype, suppressible by Tgs1^UAS.cCa/Scer\GAL4^bam.PU

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has abnormal meiotic cell cycle phenotype, suppressible by Tgs1^UAS.cCa/Scer\GAL4^bam.PU

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has male sterile phenotype, suppressible by moi^t4.Tgs1-GFP

(Cheng et al., 2020)

NOT suppressed by

Statement

Reference

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has male sterile phenotype, non-suppressible by moi^UAS.cCa/Scer\GAL4^bam.PU

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has abnormal meiotic cell cycle phenotype, non-suppressible by moi^UAS.cCa/Scer\GAL4^bam.PU

(Cheng et al., 2020)

Other

Statement

Reference

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has viable phenotype

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has male sterile phenotype

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has abnormal meiotic cell cycle phenotype

(Cheng et al., 2020)

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has female semi-sterile phenotype

(Cheng et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has testis phenotype, suppressible by Tgs1^UAS.cCa/Scer\GAL4^bam.PU

(Cheng et al., 2020)

NOT suppressed by

Statement

Reference

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has testis phenotype, non-suppressible by moi^UAS.cCa/Scer\GAL4^bam.PU

(Cheng et al., 2020)

Other

Statement

Reference

Tgs1^CB02140/Tgs1^15-11, moi^CB02140/moi^15-11 has testis phenotype

(Cheng et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tgs1^15-11

tgs1^15-11

(Cheng et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections