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General Information
Symbol
TEV\NIaT173V.RSRT.Flp1.LexAop2.Tag:M(rCd2),Tag:MYC
Species
P. Tobacco etch virus
Name
FlyBase ID
FBal0365168
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

lexAop (LexAop2) regulates expression of TEV\NIa containing the T173V mutation (to remove a predicted glycosylation site and thus make TEV\NIa active) followed by the extracellular portion of a transmembrane protein (Tag:M(rCd2)). The N-terminus has the C. elegans PAT3 signal peptide sequence for endoplasmic reticulum targeting, while the C-terminus has the Kir2.1 ER export signal to improve trafficking to the plasma membrane, followed by the P10 3' UTR. Tag:MYC has been introduced in between the TEV\NIa and Tag:M(rCd2) sequence. A removable cassette is present prior to the signal peptide, comprising Scer\FLP1 and Hsp70b and SV40 3' UTRs surrounded by RSRT recombination sites. Thus, FLP activity can remove the cassette, placing TEV\NIa-Tag:M(rCd2) under control of lexAop. (GenBank MN658788.) Used in the first (release) step of the BacTrace system.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
TEV\NIaT173V.RSRT.Flp1.LexAop2.Tag:M(rCd2),Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (1)