FB2025_01 , released February 20, 2025
Allele: Dmel\StaclΔSH3.1
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General Information
Symbol
Dmel\StaclΔSH3.1
Species
D. melanogaster
Name
FlyBase ID
FBal0365691
Feature type
allele
Associated gene
Dmel\Stacl
Associated Insertion(s)
TI{TI}StaclΔSH3.1
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Hsu et al., 2020)
gene targeting by homologous recombination
(Hsu et al., 2020)
Progenitor genotype
Associated Insertion(s)
TI{TI}StaclΔSH3.1
Cytology
Description

Sequence encoding the Stacl SH3 domain has been deleted. Sequence including a single attP site and a single loxP site have been inserted in place of the deleted sequence.

(Hsu et al., 2020)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2R:15,465,404..15,466,867 [+]
Inserted_sequence:

GTAGTGCCCCAACTGGGGTAACCTTTGAGTTCTCTCAGTTGGGGGCGTAGATAACTTCGTATAATGTATGCTATACGAAGTTAT

Comment:

Sequence encoding the Stacl SH3 domain has been deleted. Sequence including a single attP site and a single loxP site have been inserted in place of the deleted sequence. Deletion endpoints determined from junction seqiuences.

(Hsu et al., 2020)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      StaclΔSH3.1 or StaclLA00216/StaclΔSH3.1 third instar larvae exhibit decreased locomotion.

      Expression of StaclUAS.RE.EGFP under the control of Scer\GAL4da.G32 in the StaclΔSH3.1/+ (control) background leads to an increase in locomotion of third instar larvae.

      During physiological activation, StaclΔSH3.1 larvae exhibit decreased Ca[2+] transients in RP2 motor neuron type Is boutons (on muscle 4).

      StaclΔSH3.1 larvae exhibit largely decreased Dmca1D currents in RP2 motor neuron cell bodies.

      StaclΔSH3.1 larvae exhibit a decrease in neuropeptide (Dilp2-GFP) release in type I boutons (on muscle 4) upon activation of RP2 motor neuron via exposure to high potassium.

      (Hsu et al., 2020)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DstacΔSH3.1
      (Hsu et al., 2020)
      StaclΔSH3.1
      Name Synonyms
      Secondary FlyBase IDs
        References (1)