FB2025_01 , released February 20, 2025
Allele: Avic\GFPY221F.UAS.AblFRET
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General Information
Symbol
Avic\GFPY221F.UAS.AblFRET
Species
A. victoria
Name
FlyBase ID
FBal0366574
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{UAS-Abl.Y221F.FRET}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kannan et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-Abl.Y221F.FRET}
Cytology
Description

UAS regulatory sequences drive expression of a phosphorylation substrate region (in this case from the mammalian CrkII protein) that is sandwiched between ECFP and EYFP and that has been mutated to carry the Y221F amino acid substitution; this mutation renders the natural Abl phosphorylation site unphosphorylatable. The P{UAS-Abl.Y221F.FRET} transgene acts as a negative control for experiments that use the P{UAS-AblFRET} Abl activity sensor; the mutation in P{UAS-Abl.Y221F.FRET} abolishes changes in FRET in response to Abl activity (PMID:11752449).

(Kannan et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
ECFP
EYFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Avic\GFPY221F.UAS.AblFRET
      Name Synonyms
      Secondary FlyBase IDs
        References (2)