FB2025_01 , released February 20, 2025
Allele: Dmel\GrafCR57
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General Information
Symbol
Dmel\GrafCR57
Species
D. melanogaster
Name
FlyBase ID
FBal0368117
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Nature of the Allele
Progenitor genotype
Cytology
Description

A stop codon at the 28th amino acid is predicted to result in a 27aa peptide.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Nucleotide change:

G15757952T

Amino acid change:

E28term | Graf-PA; E28term | Graf-PD; E28term | Graf-PE; E28term | Graf-PG; E28term | Graf-PH; E28term | Graf-PI; E28term | Graf-PJ

Reported amino acid change:

E28term

Comment:

Reported as a stop codon at amino acid 28 leading to a 27aa Graf protein. Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

GrafCR57/GrafCR57 embryos do not show the polygonal F-actin organization in early stages of cellularization; instead there is premature contractile ring formation during early cellularization and enhanced ring constriction in mid and late cellularization, with the nuclei displaying a bottleneck appearance. Laser ablation experiments show increased contractile ring tension/contractility (increased displacement and initial recoil velocity). The slow phase of furrow extension is extended and the fast phase starts at a later time point.

External Data
Interactions
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Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)