UASp regulatory sequences drive expression of the longer isoform of dock (isoforms B, C and D) plus approximately 400bp of 3'UTR (sequence amplified from the LD42588 cDNA clone). The coding sequence is tagged at the N-terminal end with Tag:Myr(Unk) (to tether the protein to the membrane) and with Tag:HA.
Scer\GAL4VP16.mat.αTub67C, dockUASp.Tag:HA,Tag:Myr(Unk) has nurse cell ring canal phenotype, non-enhanceable by msn172/msn[+]
Scer\GAL4VP16.mat.αTub67C, dockUASp.Tag:HA,Tag:Myr(Unk) has nurse cell ring canal phenotype, non-enhanceable by msn172/msn[+]/WASp[+]/WASp1
Scer\GAL4VP16.mat.αTub67C, dockUASp.Tag:HA,Tag:Myr(Unk) has nurse cell ring canal phenotype, suppressible | partially by WASp[+]/WASp1
Scer\GAL4VP16.mat.αTub67C, dockUASp.Tag:HA,Tag:Myr(Unk) has nurse cell ring canal phenotype, suppressible | partially by Arp3515FC/Arp3[+]
Scer\GAL4VP16.mat.αTub67C, dockUASp.Tag:HA,Tag:Myr(Unk) has nurse cell ring canal phenotype, non-suppressible by msn172/msn[+]
Scer\GAL4VP16.mat.αTub67C, dockUASp.Tag:HA,Tag:Myr(Unk) has nurse cell ring canal phenotype, non-suppressible by msn172/msn[+]/WASp[+]/WASp1