FB2025_01 , released February 20, 2025
Allele: Dmel\COX4LKO
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General Information
Symbol
Dmel\COX4LKO
Species
D. melanogaster
Name
FlyBase ID
FBal0373725
Feature type
allele
Associated gene
Dmel\COX4L
Associated Insertion(s)
TI{TI}COX4LKO
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Eslamieh et al., 2022)
Mutagen
CRISPR/Cas9
(Eslamieh et al., 2022)
Progenitor genotype
Associated Insertion(s)
TI{TI}COX4LKO
Cytology
Description

Replacement of the endogenous COX4L gene with a DsRed marker.

(Eslamieh et al., 2022)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2R:5,354,091..5,354,626 [+]
Comment:

The endogenous COX4L gene is replaced with a DsRed marker. The deletion boundaries were inferred from the homology arm sequences.

(Eslamieh et al., 2022)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      COX4LKO homozygous males exhibit empty seminal vesicles and sperm bundles that fail to individualize and produce mobile sperm. Testis show a decrease in mitochondria (MitoTracker staining)

      (Eslamieh et al., 2022)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      COX4LKO has male sterile | recessive phenotype, non-suppressible by Scer\GAL4VP16.bam/COX4UAS.ORF.GW.Tag:HA

      (Eslamieh et al., 2022)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      COX4L-KO
      (Eslamieh et al., 2022)
      COX4LKO
      Name Synonyms
      Secondary FlyBase IDs
        References (1)