FB2025_01 , released February 20, 2025
Allele: Dmel\cicsgRNA.UAS
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General Information
Symbol
Dmel\cicsgRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0374044
Feature type
allele
Associated gene
Dmel\cic
Associated Insertion(s)
Carried in Construct
P{UAS-Apc,Apc2,p53,Med,Pten,cic.sgRNA}
Key Links
Transgenic product class
sgRNA
(Zipper et al., 2022)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Zipper et al., 2022)
Mutagen
in vitro construct
(Zipper et al., 2022)
Progenitor genotype
Carried in construct
P{UAS-Apc,Apc2,p53,Med,Pten,cic.sgRNA}
Cytology
Description

UAS regulates expression of a sequence containing sgRNAs targeting Apc, Apc2, p53, Med, Pten and cic, which are the orthologs of the most frequently mutated tumor suppressors in colorectal cancer patients. Each sgRNA is flanked by tRNA sequences. Note that a knockout of cic could not be verified.

(Zipper et al., 2022)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
cic
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  colorectal cancer
      CEA with Ras85DV12.cUa.UAS
      (Zipper et al., 2022)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
       

      This allele is generated as a part of the multi-gene construct P{UAS-Apc,Apc2,p53,Med,Pten,cic.sgRNA}, therefore the other mutations in that construct are included in this model.

      (Zipper et al., 2022)
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      cicsgRNA.UAS
      cicsgRNA
      (Zipper et al., 2025)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)