FB2025_01 , released February 20, 2025
Allele: Dmel\Ctl2CR70142-KO-kG4
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General Information
Symbol
Dmel\Ctl2CR70142-KO-kG4
Species
D. melanogaster
Name
FlyBase ID
FBal0375632
Feature type
allele
Associated gene
Dmel\Ctl2
Associated Insertion(s)
TI{KozakGAL4}Ctl2CR70142-KO-kG4
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{KozakGAL4}Ctl2CR70142-KO-kG4
Cytology
Description

A TI{KozakGAL4} DNA cassette has been inserted into Ctl2, replacing the coding sequence (coordinates of deleted sequence are 3R:29125414..29127984 , release 6 genome). This results in a simultaneous knock-out of Ctl2 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of Ctl2 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: CTTTCAGTAGAGAGCGATGACGG and TTGCTCAACCGGCTGCAATTGGG. The PCR check gave the expected-sized band for the right flank and longer than expected band for the left flank. The right insertion site was verified by sequencing.

(Gene Disruption Project members, 2018-)
Allele components
Component
Use(s)
Inserted element
TI{KozakGAL4}
Encoded product / tool
GAL4
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ctl2CR70142-KO-kG4
      Name Synonyms
      Secondary FlyBase IDs
        References (3)