FlyBase Allele Report: Dmel\ash1[CR70215-KO-kG4]

General Information

Symbol

Dmel\ash1^{CR70215-KO-kG4}

Species

D. melanogaster

Name

FlyBase ID

FBal0375653

Feature type

allele

Associated gene

Dmel\ash1

Associated Insertion(s)

TI{KozakGAL4}ash1^{CR70215-KO-kG4}

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{KozakGAL4}ash1^{CR70215-KO-kG4}

Cytology

Description

A TI{KozakGAL4} DNA cassette has been inserted into ash1, replacing the coding sequence (coordinates of deleted sequence are 3L:19591258..19598516 , release 6 genome). This results in a simultaneous knock-out of ash1 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of ash1 (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: TGGCTTGCCTGCTTCTAAAATGG and TAATCAATGCCTCTAACTTTAGG.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{KozakGAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

FRT