FlyBase Allele Report: Dmel\gpp[CR70526-TG4.1]

A TI{CRIMIC.TG4.1} DNA cassette has been inserted into gpp, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: TAAAAGAGGCCCCTCCTATACGG.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.TG4.1}

gene trap

RMCE target element

Encoded product / tool

GAL4

binary expression system - driver

Also carries

attP

integrase target site

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion_site

3R:6,439,362..6,439,363 [+]

(Nil et al., 2023, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of syndromic intellectual disability

CEA

(Nil et al., 2023)

CEA with gpp^XXV

(Nil et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with Df(3R)BSC193)

(Nil et al., 2023)

abnormal developmental rate (with gpp^XXV)

(Nil et al., 2023)

lethal - all die during larval stage (with Df(3R)BSC193)

(Nil et al., 2023)

lethal - all die during larval stage (with gpp^XXV)

(Nil et al., 2023)

lethal - all die during larval stage | recessive

(Nil et al., 2023)

partially lethal - majority die (with gpp^GS13895), with Scer\GAL4^{gpp-CR70526-TG4.1}

(Nil et al., 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Hsap\DOT1L^{C45G.UAS.Tag:HA}/Scer\GAL4^{gpp-CR70526-TG4.1}

(Nil et al., 2023)

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Hsap\DOT1L^{E123K.UAS.Tag:HA}/Scer\GAL4^{gpp-CR70526-TG4.1}

(Nil et al., 2023)

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Scer\GAL4^{gpp-CR70526-TG4.1}/Hsap\DOT1L^{L626V.UAS.Tag:HA}

(Nil et al., 2023)

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Hsap\DOT1L^UAS.Tag:HA/Scer\GAL4^{gpp-CR70526-TG4.1}

(Nil et al., 2023)

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Hsap\DOT1L^{K1025M.UAS.Tag:HA}/Scer\GAL4^{gpp-CR70526-TG4.1}

(Nil et al., 2023)

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Hsap\DOT1L^{E129K.UAS.Tag:HA}/Scer\GAL4^{gpp-CR70526-TG4.1}

(Nil et al., 2023)

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Scer\GAL4^{gpp-CR70526-TG4.1}/Hsap\DOT1L^{R292C.UAS.Tag:HA}

(Nil et al., 2023)

gpp^{CR70526-TG4.1}/Df(3R)BSC193 has lethal - all die during larval stage phenotype, non-suppressible by Hsap\DOT1L^{T100M.UAS.Tag:HA}/Scer\GAL4^{gpp-CR70526-TG4.1}

(Nil et al., 2023)

Other

Statement

Reference

Hsap\DOT1L^{L626V.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal - majority die phenotype

(Nil et al., 2023)

Hsap\DOT1L^{L626V.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{L626V.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{K1025M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal - majority live phenotype

(Nil et al., 2023)

Hsap\DOT1L^{K1025M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{K1025M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^UAS.Tag:HA, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E123K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal - majority die phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E123K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E123K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E129K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal - majority die phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E129K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E129K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^UAS.Tag:HA, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{T100M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{T100M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{T100M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal phenotype

(Nil et al., 2023)

Hsap\DOT1L^{R292C.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal - majority live phenotype

(Nil et al., 2023)

Hsap\DOT1L^{R292C.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{R292C.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^UAS.Tag:HA, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal phenotype

(Nil et al., 2023)

Hsap\DOT1L^{C45G.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has visible | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{C45G.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has melanotic necrosis | adult stage phenotype

(Nil et al., 2023)

Hsap\DOT1L^{C45G.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has partially lethal phenotype

(Nil et al., 2023)

Phenotype Manifest In

Other

Statement

Reference

Hsap\DOT1L^UAS.Tag:HA, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^UAS.Tag:HA, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing vein phenotype

(Nil et al., 2023)

Hsap\DOT1L^{L626V.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^{L626V.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing vein phenotype

(Nil et al., 2023)

Hsap\DOT1L^{K1025M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^{K1025M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing vein phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E123K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E129K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^{E129K.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing vein phenotype

(Nil et al., 2023)

Hsap\DOT1L^{T100M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^{T100M.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing vein phenotype

(Nil et al., 2023)

Hsap\DOT1L^{R292C.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^{R292C.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing vein phenotype

(Nil et al., 2023)

Hsap\DOT1L^{C45G.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing phenotype

(Nil et al., 2023)

Hsap\DOT1L^{C45G.UAS.Tag:HA}, Scer\GAL4^{gpp-CR70526-TG4.1}, gpp^{CR70526-TG4.1} has wing vein phenotype

(Nil et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

97194

y¹ w^*; TI{CRIMIC.TG4.1}gpp^{CR70526-TG4.1}/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

gpp^{CR70526-TG4.1}

gpp^TG4

(Nil et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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