FB2025_01 , released February 20, 2025
Allele: Dmel\GCC88CR70589-KO-kG4
Open Close
General Information
Symbol
Dmel\GCC88CR70589-KO-kG4
Species
D. melanogaster
Name
FlyBase ID
FBal0391212
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Nature of the Allele
Allele class
Progenitor genotype
Associated Insertion(s)
Caused by aberration
Cytology
Description

A TI{KozakGAL4} DNA cassette has been inserted into GCC88, replacing the coding sequence (coordinates of deleted sequence are 3R:11228191..11230580 , release 6 genome). This results in a simultaneous knock-out of GCC88 plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of GCC88 (predicted to gene trap all annotated transcripts of the gene). The deletion also removes part of CG17726. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: CCTGAGCAAACATGACAAGTTGG and CTATTTAGGATCTGTTTGTAGGG. The 5' end of the deletion associated with the insertion is 37 bp downstream of the 3' end of the CG17187 gene and may affect the proper 3' end formation of its transcripts.

Allele components
Component
Use(s)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
GCC88CR70589-KO-kG4
Name Synonyms
Secondary FlyBase IDs
    References (2)