The ubiquitous U6 promoter drives expression of three sgRNAs, all of which target RNaseZ.
abnormal developmental rate | larval stage, with Spyo\Cas9tin.4xCΔ4
decreased body size | larval stage, with Spyo\Cas9tin.4xCΔ4
decreased body weight | larval stage, with Spyo\Cas9tin.4xCΔ4
increased size | larval stage, with Spyo\Cas9tin.4xCΔ4
lethal, with Spyo\Cas9tin.4xCΔ4
lethal - all die during second instar larval stage, with Spyo\Cas9Act5C.cPa
lethal - all die during second instar larval stage | parental effect, with Spyo\Cas9αTub84B.PH
majority die during larval stage, with Spyo\Cas9tin.4xCΔ4
some die during P-stage, with Spyo\Cas9tin.4xCΔ4
embryonic/larval heart | larval stage, with Spyo\Cas9tin.4xCΔ4
RNaseZU6.3xsgRNA is rescued by RNaseZΔPAM.Tag:V5
RNaseZU6.3xsgRNA is not rescued by RNaseZTag:V5