FB2025_01 , released February 20, 2025
Allele: Avic\GFPC-Venus.UAS.Tag:PH(step)
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General Information
Symbol
Avic\GFPC-Venus.UAS.Tag:PH(step)
Species
A. victoria
Name
FlyBase ID
FBal0392364
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
PBac{UAS-VenusC-PH-GRP}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Khuong et al., 2013)
Progenitor genotype
Carried in construct
PBac{UAS-VenusC-PH-GRP}
Cytology
Description

UAS regulatory sequences drive expression of C-Venus that is tagged with Tag:PH(step).

(Khuong et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
C-Venus
Tagged with
Tag:PH(step)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies co-expressing stepPH.Scer\UAS.T:Avic\GFP-VC and stepPH.Scer\UAS.T:Avic\GFP-VN under the control of Scer\GAL4n-syb.PS show temperature-sensitive paralysis, with all flies being paralysed within 3 minutes at 38[o]C. The flies recover slowly when placed back at room temperature.

      The amplitude of the excitatory junctional current at the neuromuscular junction is significantly reduced compared to wild type in larvae co-expressing stepPH.Scer\UAS.T:Avic\GFP-VC and stepPH.Scer\UAS.T:Avic\GFP-VN under the control of Scer\GAL4n-syb.PS.

      (Khuong et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The temperature-sensitive paralysis caused by co-expression of stepPH.Scer\UAS.T:Avic\GFP-VC and stepPH.Scer\UAS.T:Avic\GFP-VN under the control of Scer\GAL4n-syb.PS is completely suppressed if the flies are also co-expressing both Hsap\MTORFRB.Scer\UAS.T:Uuuu\Lyn11 and Hsap\FKBP1AFKBP.Scer\UAS and are fed rapamycin.

      The reduced amplitude of the excitatory junctional current at the neuromuscular junction which is seen in larvae co-expressing stepPH.Scer\UAS.T:Avic\GFP-VC and stepPH.Scer\UAS.T:Avic\GFP-VN under the control of Scer\GAL4n-syb.PS is completely suppressed if the flies are also co-expressing both Hsap\MTORFRB.Scer\UAS.T:Uuuu\Lyn11 and Hsap\FKBP1AFKBP.Scer\UAS and are fed rapamycin.

      (Khuong et al., 2013)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Avic\GFPC-Venus.UAS.Tag:PH(step)
      stepPH.Scer\UAS.T:Avic\GFP-VC
      stepPH.UAS.C-Venus
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0392319
      • FBal0285587
      References (3)