FlyBase Allele Report: Hsap\UBA5[UAS.cBa]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Hsap\UBA5^UAS.cBa

General Information

Symbol

Hsap\UBA5^UAS.cBa

Species

H. sapiens

Name

FlyBase ID

FBal0397639

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

PBac{UAS-hUBA5.B}

Key Links

Transgenic product class

(Celniker and Bellen, 2017-)

(Celniker and Bellen, 2017-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Celniker and Bellen, 2017-)

(Celniker and Bellen, 2017-)

Mutagen

in vitro construct

(Celniker and Bellen, 2017-)

Progenitor genotype

Carried in construct

PBac{UAS-hUBA5.B}

Cytology

Description

UASt regulatory sequences drive expression of the Hsap\UBA5 open reading frame. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF was amplified from the IOH9860 clone. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Celniker and Bellen, 2017-)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

recombinase target site

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates developmental and epileptic encephalopathy 44

modeled by Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\UBA5^UAS.cBa/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of lethal - all die before end of first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of majority die during embryonic stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa/Scer\GAL4^{Uba5-CR00497-TG4.2} is a suppressor of some die during first instar larval stage | male phenotype of Uba5^{CR00497-TG4.2}

(Pan et al., 2023)

Scer\GAL4^da.PU/Hsap\UBA5^UAS.cBa is a suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

Scer\GAL4^Act5C.PI/Hsap\UBA5^UAS.cBa is a suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

NOT Suppressor of

Statement

Reference

Scer\GAL4^apolpp.PB/Hsap\UBA5^UAS.cBa is a non-suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

Scer\GAL4^repo/Hsap\UBA5^UAS.cBa is a non-suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

Scer\GAL4^Mef2.PR/Hsap\UBA5^UAS.cBa is a non-suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa/Scer\GAL4^elav.PLu is a non-suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

Scer\GAL4^repo, Hsap\UBA5^UAS.cBa, Scer\GAL4^elav.PLu is a non-suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

Other

Statement

Reference

Hsap\UBA5^UAS.cBa, Scer\GAL4^Act5C.PI, Uba5^KO/Uba5[+] has viable | female phenotype

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa, Scer\GAL4^da.PU, Uba5^KO/Uba5[+] has viable | female phenotype

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa, Scer\GAL4^elav.PLu, Uba5^KO/Uba5[+] has viable | female phenotype

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa, Scer\GAL4^apolpp.PB, Uba5^KO/Uba5[+] has viable | female phenotype

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa, Scer\GAL4^repo, Uba5^KO/Uba5[+] has viable | female phenotype

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa, Scer\GAL4^Mef2.PR, Uba5^KO/Uba5[+] has viable | female phenotype

(Pan et al., 2023)

Hsap\UBA5^UAS.cBa, Scer\GAL4^elav.PLu, Scer\GAL4^repo, Uba5^KO/Uba5[+] has viable | female phenotype

(Pan et al., 2023)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

602405

y¹ w^*; PBac{UAS-hUBA5.B}VK00037/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Hsap\UBA5^UAS.cBa

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections