FB2025_01 , released February 20, 2025
Allele: Disc\RFPtdT.UASp.F-Tractin
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General Information
Symbol
Disc\RFPtdT.UASp.F-Tractin
Species
D. sp.
Name
FlyBase ID
FBal0397717
Feature type
allele
Associated gene
Disc\RFP
Associated Insertion(s)
Carried in Construct
P{UASp-F-Tractin.tdTomato}
Also Known As
UASp-F-Tractin-tdTomato
Transgenic product class
inframe_deletion
(Spracklen et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Spracklen et al., 2014)
Mutagen
in vitro construct
(Spracklen et al., 2014)
Progenitor genotype
Carried in construct
P{UASp-F-Tractin.tdTomato}
Cytology
Description

UASp regulatory sequences drive expression of tdTomato that is tagged at the N-terminal end (via a GGSGSDPPVAT linker) with F-Tractin.

(Spracklen et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
tdTomato
Tagged with
F-Tractin
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      During follicle stage 9 neither weak expression of Rnor\Itpka9-40.Scer\UAS.P\T.T:Disc\RFP-tdTomato in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16, or expression in the soma under the control of Scer\GAL4c355 causes any apparent defects in F-actin within either the soma or the germline. When Rnor\Itpka9-40.Scer\UAS.P\T.T:Disc\RFP-tdTomato is expressed using strong germline drivers (Scer\GAL4mat.αTub67C.T:Hsim\VP16 or Scer\GAL4osk.T:Hsim\VP16) aberrant early F-actin filaments and, at a low frequency, aggregates are sometimes observed. S10B follicles expressing Rnor\Itpka9-40.Scer\UAS.P\T.T:Disc\RFP-tdTomato are generally normal in morphology and F-actin structures.

      (Spracklen et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The plasmid used to create this line was from Michael Schell (Johnson and Schell, 2008 - PMID:PMID: 19846664).

      (Spracklen et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Disc\RFPtdT.UASp.F-Tractin
      Rnor\Itpka9-40.Scer\UAS.P\T.T:Disc\RFP-tdTomato
      Rnor\Itpka9-40.UASp.tdTomato
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0397500
      • FBal0298817
      References (6)