FB2025_01 , released February 20, 2025
Allele: Dmel\ApcCR71210-KO-kG4-X
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General Information
Symbol
Dmel\ApcCR71210-KO-kG4-X
Species
D. melanogaster
Name
FlyBase ID
FBal0399771
Feature type
allele
Associated gene
Dmel\Apc
Associated Insertion(s)
TI{KozakGAL4}ApcCR71210-KO-kG4-X
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{KozakGAL4}ApcCR71210-KO-kG4-X
Cytology
Description

A TI{KozakGAL4} DNA cassette has been inserted into Apc, replacing the coding sequence (coordinates of deleted sequence are 3R:28833237..28841693 , release 6 genome). This results in a simultaneous knock-out of Apc plus a knock-in of GAL4. The cassette is not predicted to trap Apc or express GAL4 because the 5' end of the insertion is in an intron in the 5' UTR. The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: GGCTGCCGCCACCTGAATGTTGG and TGGTGGGCGCGCGGAAATAGAGG.

(Gene Disruption Project members, 2018-)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
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      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
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      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ApcCR71210-KO-kG4-X
      Name Synonyms
      Secondary FlyBase IDs
        References (1)