FlyBase Allele Report: Dmel\amls[RNAi.10xUAS.3-ORF]

General Information

Symbol

Dmel\amls^{RNAi.10xUAS.3-ORF}

Species

D. melanogaster

Name

FlyBase ID

FBal0404336

Feature type

allele

Associated gene

Dmel\amls

Associated Insertion(s)

Carried in Construct

M{10xUAS-amls.RNAi.3-ORF}

Key Links

Transgenic product class

RNAi_reagent

(Reim et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Reim et al., 2014)

Mutagen

in vitro construct

(Reim et al., 2014)

Progenitor genotype

Carried in construct

M{10xUAS-amls.RNAi.3-ORF}

Cytology

Description

10xUAS regulatory sequences drive expression of a short hairpin RNA (shRNA) that targets the als open reading frame (target site: CGGACGAAAGCCTGGATTTTA).

(Reim et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

amls

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^hh.PU

(Reim et al., 2014)

abnormal size | adult stage, with Scer\GAL4^pnr.PU

(Reim et al., 2014)

abnormal size | adult stage | somatic clone, with Scer\GAL4^Act5C.PU

(Reim et al., 2014)

lethal - all die during P-stage, with Scer\GAL4^nub.PU

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^en.PU

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^ey.PU

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^hh.PU

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^pnr.PU

(Reim et al., 2014)

visible | adult stage, with Scer\GAL4^vg.PU

(Reim et al., 2014)

visible | adult stage | somatic clone, with Scer\GAL4^Act5C.PU

(Reim et al., 2014)

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr.PU

(Reim et al., 2014)

embryonic/first instar larval cuticle | embryonic stage, with Scer\GAL4^da.PU

(Reim et al., 2014)

eye, with Scer\GAL4^ey.PU

(Reim et al., 2014)

mechanosensory chaeta, with Scer\GAL4^pnr.PU

(Reim et al., 2014)

scutellum, with Scer\GAL4^pnr.PU

(Reim et al., 2014)

ventral denticle belt | embryonic stage, with Scer\GAL4^da.PU

(Reim et al., 2014)

wing, with Scer\GAL4^en.PU

(Reim et al., 2014)

wing, with Scer\GAL4^hh.PU

(Reim et al., 2014)

wing margin, with Scer\GAL4^vg.PU

(Reim et al., 2014)

wing margin | somatic clone, with Scer\GAL4^Act5C.PU

(Reim et al., 2014)

wing sensillum | somatic clone, with Scer\GAL4^Act5C.PU

(Reim et al., 2014)

wing vein | somatic clone, with Scer\GAL4^Act5C.PU

(Reim et al., 2014)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, amls^{RNAi.10xUAS.3-ORF} has visible | adult stage phenotype, suppressible by Hsap\UBXN6^UAS.Tag:HA, Scer\GAL4^ey.PU

(Reim et al., 2014)

Scer\GAL4^nub.PU, amls^{RNAi.10xUAS.3-ORF} has increased mortality during development phenotype, suppressible by Scer\GAL4^nub.PU/Ubi-p5E^HMS01881

(Reim et al., 2014)

Scer\GAL4^ey.PU, amls^{RNAi.10xUAS.3-ORF} has visible | adult stage phenotype, suppressible by Ubi-p5E^HMS01881/Scer\GAL4^ey.PU

(Reim et al., 2014)

Scer\GAL4^nub.PU, amls^{RNAi.10xUAS.3-ORF} has increased mortality during development phenotype, suppressible by CSN6^UAS.ORF, Scer\GAL4^nub.PU

(Reim et al., 2014)

Scer\GAL4^ey.PU, amls^{RNAi.10xUAS.3-ORF} has visible | adult stage phenotype, suppressible by CSN6^UAS.ORF, Scer\GAL4^ey.PU

(Reim et al., 2014)

NOT Enhancer of

Statement

Reference

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^ey.PU is a non-enhancer of increased size | adult stage phenotype of upd1^GMR.PB

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^ey.PU is a non-enhancer of visible | adult stage phenotype of upd1^GMR.PB

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^GMR.PU is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cUa

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^GMR.PU is a non-enhancer of decreased size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cUa

(Reim et al., 2014)

Suppressor of

Statement

Reference

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^nub.PU, arr^{UAS.cUa.Tag:HA}

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of increased size | adult stage phenotype of Scer\GAL4^nub.PU, arr^{UAS.cUa.Tag:HA}

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^nub.PU, sgg^RNAi.UAS.cUa

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of decreased size | adult stage phenotype of Scer\GAL4^nub.PU, sgg^RNAi.UAS.cUa

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^C-765 is a suppressor of visible | adult stage phenotype of Notum^GD1719, Scer\GAL4^C-765

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^C-765 is a suppressor of decreased size | adult stage phenotype of Notum^GD1719, Scer\GAL4^C-765

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^ey.PU, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of wg^hs.2sev

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^ey.PU, Scer\GAL4^GMR.PU is a suppressor | partially of decreased size | adult stage phenotype of wg^hs.2sev

(Reim et al., 2014)

NOT Suppressor of

Statement

Reference

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^nub.PU is a non-suppressor of visible | adult stage phenotype of E(spl)m8-HLH^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^nub.PU

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^ey.PU is a non-suppressor of increased size | adult stage phenotype of upd1^GMR.PB

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^ey.PU is a non-suppressor of visible | adult stage phenotype of upd1^GMR.PB

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cUa

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^GMR.PU is a non-suppressor of decreased size | adult stage phenotype of Scer\GAL4^GMR.PU, egr^UAS.cUa

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^nub.PU, slmb^HMS00838

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a non-suppressor of decreased size | adult stage phenotype of Scer\GAL4^nub.PU, slmb^HMS00838

(Reim et al., 2014)

Other

Statement

Reference

Scer\GAL4^nub.PU, TER94^{UAS.ORF.Tag:HA}, amls^{RNAi.10xUAS.3-ORF} has visible | adult stage phenotype

(Reim et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, amls^{RNAi.10xUAS.3-ORF} has eye phenotype, suppressible by Ubi-p5E^HMS01881/Scer\GAL4^ey.PU

(Reim et al., 2014)

Scer\GAL4^ey.PU, amls^{RNAi.10xUAS.3-ORF} has eye phenotype, suppressible by CSN6^UAS.ORF, Scer\GAL4^ey.PU

(Reim et al., 2014)

Scer\GAL4^ey.PU, amls^{RNAi.10xUAS.3-ORF} has eye phenotype, suppressible by Hsap\UBXN6^UAS.Tag:HA, Scer\GAL4^ey.PU

(Reim et al., 2014)

NOT Enhancer of

Statement

Reference

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^ey.PU is a non-enhancer of eye phenotype of upd1^GMR.PB

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cUa

(Reim et al., 2014)

Suppressor of

Statement

Reference

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^nub.PU, arr^{UAS.cUa.Tag:HA}

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, arr^{UAS.cUa.Tag:HA}

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, sgg^RNAi.UAS.cUa

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^nub.PU, sgg^RNAi.UAS.cUa

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^C-765 is a suppressor of wing vein | ectopic phenotype of Notum^GD1719, Scer\GAL4^C-765

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^C-765 is a suppressor of wing phenotype of Notum^GD1719, Scer\GAL4^C-765

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^ey.PU, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of wg^hs.2sev

(Reim et al., 2014)

NOT Suppressor of

Statement

Reference

amls^{RNAi.10xUAS.3-ORF}, Scer\GAL4^nub.PU is a non-suppressor of wing vein | absent phenotype of E(spl)m8-HLH^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^nub.PU

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^nub.PU is a non-suppressor of wing phenotype of Scer\GAL4^nub.PU, slmb^HMS00838

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of upd1^GMR.PB

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF}/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cUa

(Reim et al., 2014)

Other

Statement

Reference

Scer\GAL4^nub.PU, TER94^{UAS.ORF.Tag:HA}, amls^{RNAi.10xUAS.3-ORF} has wing margin phenotype

(Reim et al., 2014)

E(spl)m8-HLH^{UAS.ORF.GW.Tag:HA}, Scer\GAL4^nub.PU, amls^{RNAi.10xUAS.3-ORF} has wing margin phenotype

(Reim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

amls^{RNAi.10xUAS.3-ORF} is partially rescued by amls^{UAS.3-ORF-RR.N-Tag:HA}/Scer\GAL4^vg.PU

(Reim et al., 2014)

amls^{RNAi.10xUAS.3-ORF} is partially rescued by amls^{UAS.3-ORF-RR.N-Tag:HA}/Scer\GAL4^ey.PU

(Reim et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

als^{RNAi.10xUAS.3-ORF}

amls^{RNAi.10xUAS.3-ORF}

Name Synonyms

Secondary FlyBase IDs

References (1)

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