FlyBase Allele Report: Dmel\Mad[RNAi.UAS.cUa]

General Information

Symbol

Dmel\Mad^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0407452

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

Carried in Construct

P{UAS-Mad.RNAi.U}

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Mad.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of a Mad RNAi construct.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mad

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^A9

(Wang et al., 2018)

hyperplasia, with Scer\GAL4^GMR24H06

(Guo et al., 2013)

hyperplasia | heat sensitive, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Guo et al., 2013)

visible, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

visible | adult stage, with Scer\GAL4^A9

(Wang et al., 2018)

Phenotype Manifest In

adult posterior midgut epithelium, with Scer\GAL4^GMR24H06

(Guo et al., 2013)

adult posterior midgut epithelium | heat sensitive, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Guo et al., 2013)

wing, with Scer\GAL4^A9

(Wang et al., 2018)

wing, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

wing margin bristle | ectopic, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

wing sensillum | ectopic, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

wing vein, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

wing vein L3, with Scer\GAL4^A9

(Wang et al., 2018)

wing vein L4, with Scer\GAL4^A9

(Wang et al., 2018)

wing vein L5, with Scer\GAL4^A9

(Wang et al., 2018)

Detailed Description

Statement

Reference

Expression of Mad^TRiP.cUa under the control of Scer\GAL4^A9 results in the loss of distal parts of the L3, L4 and L5 wing veins and a decrease in wing size.

(Wang et al., 2018)

Expression of Mad^{dsRNA.Scer\UAS.cUa} under the control of both Scer\GAL80^ts.αTub84B and Scer\GAL4^esg-NP5130 for 8 days leads to a dramatic increase in mitotic cell numbers in the posterior midgut as compared with sibling controls.

Expression of Mad^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^GMR24H06 for 8 days leads to a dramatic increase in mitotic cell numbers in the posterior midgut as compared with sibling controls.

Expression of Mad^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^{Myo31DF-NP0001} has no effect on mitotic cell numbers in the posterior midgut as compared to sibling controls.

(Guo et al., 2013)

Expression of Mad^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^sca-537.4 always results in a mutant wing phenotype (even if the flies are also expressing Mad^Scer\UAS.cNa). The wings are smaller than normal show loss of vein tissue. Many wings also have ectopic sensilla on the anterior crossvein, L1 and L3 veins and have dorsal chemosensory bristle duplications.

(Quijano et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mad^RNAi.UAS.cUa, Scer\GAL4^A9 has abnormal size | adult stage phenotype, suppressible | partially by anchor^GD3613, Scer\GAL4^A9

(Wang et al., 2018)

Mad^RNAi.UAS.cUa, Scer\GAL4^A9 has visible | adult stage phenotype, suppressible | partially by anchor^GD3613, Scer\GAL4^A9

(Wang et al., 2018)

Suppressor of

Statement

Reference

Mad^RNAi.UAS.cUa, Scer\GAL4^A9 is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Mad^RNAi.UAS.cUa, Scer\GAL4^A9 is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mad^RNAi.UAS.cUa, Scer\GAL4^A9 has wing phenotype, suppressible | partially by anchor^GD3613, Scer\GAL4^A9

(Wang et al., 2018)

Suppressor of

Statement

Reference

Mad^RNAi.UAS.cUa, Scer\GAL4^A9 is a suppressor | partially of wing vein phenotype of Scer\GAL4^A9, anchor^GD3613

(Wang et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The thickened wing vein phenotype of adults expressing anchor^GD3613 under the control of Scer\GAL4^A9 is suppressed by addition of Mad^TRiP.cUa. The decreased wing size phenotype of adults expressing Mad^TRiP.cUa under the control of Scer\GAL4^A9 is suppressed by addition of anchor^GD3613.

(Wang et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Mad^RNAi.UAS.cUa

Mad^TRiP.cUa

Mad^VDRC.cUa

Mad^{dsRNA.Scer\UAS.cUa}

Mad^{dsRNA.UAS.cUa}

Name Synonyms

Secondary FlyBase IDs

FBal0268587
FBal0341671
FBal0350397

References (9)

Report Sections

Open Close