FB2025_05 , released December 11, 2025
Clone: Dmel\AT13284
Open Close
General Information
Symbol
Dmel\AT13284
Species
D. melanogaster
Name
AT13284
FlyBase ID
FBcl0012723
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG13088
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
  • chimeric clone
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage | male
    testis
    (Stapleton et al., 2002)
    seminal vesicle
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,915
    NCBI
    BT003644
    Full Length sequence dataSequence Downloader
    TTTTTTTAAGATGAACCACTCGTAGCTTTTGTATTTTATGCATATATATATATATATAGGTACAGCAAATGTCTGCATAG
GTGCGCTAAATGTATGTTCTAAAAGTAAGCCAAAATATTATAAACTGTGCAATAAGTATTATCTTTCATCATTTCAGAAG
AAGCTAACGAACGGCGATCCGCTGCCGGACCCCAATAGAAATATGTATATAGCGCCATGCAAAGAGAATTATAACCAAGA
AATTAAGAACAAATACGATTTATCCATAGTGATACGTGCTAAACTCCTACGCGTTTATGACCCCGTGCGAAAATCCGCCG
TCCTCTCGGATCCTCCGCAAAAAGGAACAAAATGTGTGCAGATGTGGGTGGTGTGCATTTCTGCGGTGCGGTTGTGTCTG
AGTGGTTGTGCAATGCTTTCCCATCCTCCCAAGTCCTGTTCGGTGAACTTAGACCTCGGTGACGGGGCGCTTCTCGATCC
AGGCCTTGATCGGTTCCAAAGCGTTGACGGCATCCACGACTCCGCGCAGAGCTGGGTAGGGTTCCAACAGATCGCGCTTG
ACCATGTAGTTCATGTAGTCGGTGATGCCTGCGAAGTAGACGTCGGCCCAGGTCAGCTTGCCCAGAGCCAGGTGACCATC
GTTGTCCTTGACGGTCTGCTCGAGCTTCTCCAGGTAGAATGGAATGACCTCCGCATTCAGGGTGACCAACTTCTTCTCTT
TAATCTCGTCCTCCGGCTCGTACGAGACGACTGCAATTTTTAGACGGAAGTCATTGATGGTGTCAACGACAATGTCGATC
TGCAGATCCTCCCACGGAGTAGCGCCGCACAGGCCGACAGTCTTGGCCAGGAATCGAGCCATCGAAATGCTCTGGTGCAC
ACGCTTGCCGTCCACCTCCAGCACGGGCATCTGTCCCATGGGCATGGTGGGCTTCAGGGCTGGCCACTCGTCGCGGGTGA
CGCGCACATCCTCGTACTCCTGGTTGCCGTAGGCGAACAGGTAGCGCAGGGGCTCGGCCAACGCCTTCACGTTGAAGTAG
AAGAGCGTGTAGCTGTGCTTGATGGGCTCGGCGGGCTCTGCTGGTGGAGCGGCCTCTCCGCCCTCGACGGCACCCTCGGC
TCCCTCGGCGGGTGGTGGCGCCTCTCCCTCGGCTGGTGGTGCTCCCTCAGCTGGTGGTGCTTGTGCTTCATCGGCCATCT
CTGCGCTTTCTCAATGCAATTCCCTCAACAAAGAATACGAAATCAGCTGGGCACTTGGGCTCTCGGGTCTTCAATTCGCT
AATAAGCGTTCACTTAAATCGTGGGCGAAGGAATAGCGTTCGGTTCGATTCGCACTCTCTTTCCGTTACTCTCTACTCTC
ACTCTCTCCAGCCACTGGGAAGCTATCACTGAACCGATCAAGCATGCAAACAAACAGCTGACAGTATTTGTTTTTGGTAA
TCTCGTTTTGAACAAAAATTTCGATGTGAGATGGAGCAAATAAATGAAGATGTTTGGATAGATATACTAAAGTACCTACC
AATGAGGGATCAGTTGTCGCTGGTCGAAGTCAACGAGAATATATCGGCATACGTGAAGTATCATTGGAGTCATCTCAAAA
CAGTGACTCTGACCCGCGAGGATCTGGATTTTCTGGATCGGAATAACAAGCTGATGCACGAATGTCTGGGTGGCTGGTCG
GCAACGGTGGAGCGTTTGAATTTGCAAAGTGCCAGCAGTGATCTGCTGAGAAAGTGGACGGAATACGACTTTCCCCACCT
CCGATCGCTCGACTGCCACATGGACTACAATCTCGAGGAGGCGGACGAGGAAACCCTTCTGCTAACCGAGCTGTTCCCGC
TCTTGACACGGCTGTCACTCAATAGCAGCACAACAGGTCGTTACTTGTGGCACTGGAAGCAACTAAGGGAGTTGAATCTC
ACCTGGTGTGAGTACCTGGACCCAGACCACTTCGAGGAGATCTTTGGCAACCTACAGCTGACCAAGCTGACTATGCTCTA
TTACGGATATAACGTGAACCTAGGCGAAAAAGTTGTGGACATAACGCGATGTACCACGCTTGAAGAGCTGCACATCGATG
ACCACCATTTGCTGGGTGATTTCCTGCCCCGACTGATGAATCTGCCGCACTTCCGCCGCTTGGCCTTTTACACGCGAGAC
TACTATGAGTATTTGCTAGGTTCGGTGGCCAGGCACAAGCCGCTGAAGGTTCAATCGCTTCTCTTCAACGACTCTTTCTG
GAGCAGTGAACGGGTGGCGGGAAGTATACTGCACATGACCAATCTTCGTCGGCTGGTCCTGCAGGAGGATGACATCGATG
CGCAGCAGCTGCACACCATCTGCCACAAATTGCCAAACTTGGAGGAGCTACACCTTTTGGCGATGCGAGAAGTTCCGTCT
CCCAGCCACCTGTGGAACTCTATAGGTCACTGCCATCTTCTTAGGATTCTTAACCTATCGTCTACCAAGCTGGTGGACCT
CCGAAGCTCCTGCCTGACAAAAGTTCTGCTAAGTCGCAGGATTCCACTGACCCTGCATCTCCACAACACTGGATTGGATC
CTAGTAAAGTTCTACGGGATTTTGATAGTGCCTCCTTGAAAATCTCCTTTGAGCCGATCCATTTGAATATTTGGAGTCCG
CGATTTGTGGAAATCGAATTCAGTCCAGACTCGTAGTAGTCATTTGAAAAGCCTTAAATGACATTTCTACCTCAATCCGT
CCGGTATACGAATATATATGTAGATGGAGATCCAAATGATATATCCTGAGTAAAATGTTGTACCCTTTGCAAGGGTATAG
AGTCAAATAGCTTAACAAAACCACAAAATCTTTTATCTGTCTACCTACCAAAATCGTATTAAATTCAAGAAAATAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: AT_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

    (Stapleton et al., 2002)
    Sample preparation

    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

    Protocol

    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    AT
    (Stapleton et al., 2002)
    AT_cDNA
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)