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General Information
Symbol
Dmel\AT26944
Species
D. melanogaster
Name
AT26944
FlyBase ID
FBcl0026259
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      5' Sequence
      Total bases
      327
      NCBI
      5' sequence dataSequence Downloader
      CCTCGTGCCGGGAAAAGGTGTGAAAAGCGTATACTGTGAGCCATGGACATCGATTTGAGTCTGGACGAGATTATAGAGCG
      GAAGCGCGGGCAACGCGGCTATCGTCCCCTCGACAGAAAGTTCAATGCGAACTTCTCCAAGCCGCGCCAGCTGCTGAACA
      AGCCTTTTTTCAAGCCATCGGGTGCCCGAGGATCTCACTCTGATACGCCGGCAAAGCGCACCTTCGATGCCAGAAACACG
      ATCGCCGCGAAGACACGGGCCAAGATAGCCGATGCCCGGGATCTGATCACCAAGAGCGTGCGCGACTCGGGCATNGATGC
      CCGCCAG
      
      Library Information (1)
      Library: AT_cDNA
      Description
      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.
      Sample preparation
      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.
      Protocol
      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.
      Comment
      Reported As
      Symbol Synonyms
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)