FB2019_05, released Oct 15, 2019

Clone: Dmel\AT13846

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General Information
Symbol
Dmel\AT13846
Species
D. melanogaster
Name
AT13846
FlyBase ID
FBcl0032929
Feature type
cDNA_clone
Computed gene(s)
Dmel\P5CDh2
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      pOTB7
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Stapleton et al., 2002)
      testis
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      668
      NCBI
      BF499315
      5' sequence dataSequence Downloader
      CAAAAGCTTGAGCCCTTGTCCTCAGGATATAGAAAGTATATGGGAACTTTTACCGTCTCGCTGACATGAACGATCTCGGT
GTGACTAATTAGACGGATGGCAGCCAGTCCATAGAAGAGCATTGGGCATACACCTTTTTCGAGTTACCACGGACCTATGT
AGCATAATCCGTTTACTGGACTTACCCAAATTAATCACCTTAAGGAAGCGAAAGAACTTGTCCTCCAGCCAAAGATCCTG
CACCTCCCTCTTGCTACAGTGGGTCCAATTAAAGTGAGACATTCCCCTCTGATCGTTGGCCACATCGTGAAACTAGACTT
GGAGCATATTTCTCATTAGATCTGGTGAAAATATTGGGGCGGAAAAAGGGGAGTCTTACCTTCACGAAATATGATGTCCA
AGGTGGCTCATGGCATTGTTTCAAATATGCGGTCAATACTTCCGAGGCTCGAGGCTTCAGTAAAAGGCGGCCTCGTGTCT
GTTAGAAGTACCAGCAAGAGACCCGAGCTATCCGAGCACTTTACGCTGGATGAGATGGTGGCCAACGAGAAGCTGCTTCA
GTATGACGAGGGCTCAAAGGAACGCACGGAACTGGAAACCGCCCTGCTGGGCGTTCTCAATAGGGTAGAGCACGTTCCCA
TCGTTATTAAACGGCCAGAGTTCCAGGC
      Library Information (1)
      Library: AT_cDNA
      Description
      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.
      (Stapleton et al., 2002)
      Sample preparation
      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.
      Protocol
      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.
      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)