Open Close
General Information
Symbol
Dmel\AT07276
Species
D. melanogaster
Name
AT07276
FlyBase ID
FBcl0035909
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
unspliced intron
FlyBase assessment
  • chimeric clone
Library
AT_cDNA
  • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    4,302
    NCBI
    Full Length sequence dataSequence Downloader
    CCGATTATCTGATTAATGAGTAAAATTACTGAAATCCAAATTTCTAGTGTATCAAGCGCCGATTACCGAATAGATTTGCC
    ACTCAAAATCGTTTCGCTAGTCCAGCTTTGCAACGGAAGGCACTCGCAAAGGCGCCGTAAGATGCGCGATTGGCCGGATG
    TTATGCTGTTATGAGCCAGCACACTTCGCAGTACGTTTTCAACTCCAAGGAAGACTACAACGTAATCCTAGACAACATGA
    GTCGCGAGTTCGAAGAGCGGTGGAATCACCAGACCCAGTCGCCCTACACCAATCTGGAAAACTATATAACCCGGGCCGTC
    CTCGGCAACGGAAGTTTCGGCACTGTGATGCTGGTCAGGGAGAAGAGTGGCAAGAACTACTATGCCGCCAAGATGATGAG
    CAAGGAGGATCTGGTGCGGCTGAAGCAGGTGGCCCACGTACACAACGAGAAGCACGTCCTGAACGCCGCCCGATTCCCGT
    TCCTCATCTACCTGGTGGACTCGACCAAGTGCTTCGACTATCTCTACCTAATCCTCCCGCTGGTCAATGGAGGAGAGCTG
    TTCAGTTACCATCGCAGGTGAGTATTCCCTTTCTGGAGTGCTCATTTCATCTGATCACAGACTGGGTTCCAACGGGTTTC
    CCCCCCCCATAAAAAAGAGTTCGCAAGTTCAACGAGAAGCACGCCAGATTCTATGCCGCCCAGGTGGCACTTGCTCTTGA
    GTATATGCACAAGATGCACCTCATGTACCGTGATCTGAAGCCGGAGAACATTCTGCTCGATCAGCGCGGCTATATCAAAA
    TAACGGACTTCGGTTTCACTAAGCGTGTGGATGGCCGCACATCAACGCTGTGTGGAACCCCGGAATACTTGGCCCCGGAG
    ATTGTTCAACTCCGGCCCTACAACAAATCGGTGGACTGGTGGGCCTTTGGTATCCTAGTGTACGAGTTTGTGGCAGGGCG
    GTCTCCCTTTGCCATTCACAATCGAGATGTAATCCTGATGTACTCCAAGATCTGCATATGCGACTACAAGATGCCCTCAT
    ACTTTACGTCCCAGCTGAGGAGCCTTGTCGAGAGCCTCATGCAGGTGGACACCTCAAAGCGTTTAGGAAACTCGAACGAC
    GGCTCCAGCGACGTGAAGAGTCATCCGTGGTTCCAGGGCGTAGATTGGTTTGGCATTCTCAACCAGGAAGTCACCGCCCC
    CTACCAGCCCACCATTTCCGGCGCCGAAGATCTGTCGAACTTCGAGAACTTCGAGTTCAAGGATCGGTACAAGTCCCGAA
    TAAACCGCCATCCCGAATTGTTTGCGAATTTTTAAATGTCAATGTGTCCTTCGAAATCGTCGTTTTCTTTAGTGTTTGCT
    TAATTTCAAGGGGTGCTATCGTGATTGGATTTTGTAAAAGTAGTTTTCGCAATACAGCTCTCTTATTACATTCAAAAAAA
    AAAAAAAAAAACTCGAGACTAGTTCCTCGTGCCGAATTCGGCACGAGGAAAACTTACAGGGGCAAACCAAAACCGCTTCC
    ATCTCTGATCCACATTTACCTTTGCAGCAATTTATTGTATGAGTCAAAAAACTGAAAGACCAGTGCTATCGGGTCAACGC
    ATCAAGACCAGAAAAAGAGATGAAAGAGAAAAATATGACCCAACGGGATTCCGTGATGCGGTCATTGCTGGTCTCGAAAA
    AACTGAAGGCGACCTGGAACAAATCTCTAAATATCTAGACAGCGCGGGCAACAAGCTCGACTATCGTCGCTACGGTGAAG
    TTCTCTTTGACATATTGATTGCAGGAGGTCTACTAGTGCCTGGTGGTTCTATATCTCAGGATGGCGAAAAGCCGCGCACA
    AGCTACTGCATCTTCGATGCGCCCGAGAGCAGGTATTTGTCAAGCTCATCAGACGGTACAAGTACCTTGAGAAAATGTTC
    GAAGAGGAGATGGGAAAGGTTCTATTATTCGTAAAGGGCTTTACTCCTAGTGAACGCATCAAGCTTGCGCGTATGACAGC
    GCTCTGGTTAGTCAACGGTTCTGTACCGCCAAATGTTTTACTGGTACTAAACAACGAGCACCTAATCAAGGATGGCATTG
    CATTGGAGTTTCTACTAGAGCTGTTCCAGACGTTCAAGCAAGAGAAGGGCATTGCATATCTGATTCAGGCGCTTAAGAAG
    GGTGGACTGGAAAGCAAACTTATGGACTTTTTCCCACCGAACAAACGTACTGAGGAATATTTTAAACAAGTTTTTCTTGA
    CAAAGAACTTAACGAGATCGTCAAGCTACATAAGGCTCAGGCTAGCCAGGAGGCGAAGCGTGAGTTGCAGCAGGCGCTCA
    TCGATGATATCAACGATGAGAAGCCATATAATGAGATAACCTCAGACATTAAGGACTTCTCGCAGCGCACCAACATTCCC
    GATCATGAAATAATTGTTATTATTTGGTCCACCATCATGTCATTGGGCGAATGGAACAAGAAGGAGGAGCTGGTTACCGA
    TCAGGCTGTTCGTCATCTGAAGAACTACTGCCCACTGTTGCAGGCTTTCGCCTCGACCGACCGTTCGGAATTGGCCCTAA
    TCTTGAAGGTGCAGGAATTCTGCTACGAGAACATGAACTTCATGAAGGCCTTCCAGAAAATCATTTTGTTGTTCTACAAG
    ACCGAGGTCCTGTCCGAGGAGATCATCTTGCGCTGGTACAAGGAGGGCCACTCAAACAAGGGCAAAATGCATTTCCTTGA
    ACAAATGCGAAAGTTTGTCGAATGGCTTCAGTCAGCCGAAGAAGAATCCGAATCTGAGGACGAACAAAAGAATGGCGAGT
    AAGAACTTGGTTGATGGCTAAATGAGGATGAACACAAACGGAGCCGCCCTTTAGCATCCCCATCCAAAAATTCGAAACCA
    GCACCTGCAACAGCCAGTCGAACTATATATAAAACTCACCCCTATGAATTAATGTCATTTTGAAAACCCTCGTGCCGAAT
    TCGGCACGAGGTCAGTTTTGAGTTCACACTGAGAGAAAAGTAGCGAAAATTTAGTGAACCGAATCAAAATTTTGTGCATT
    TGTGCGGCCGCCGCTGGCATCGTGATATAACAGGAGTTGCCGCTCGACTTTCTCTACTTACTCTCCCTCTCTCGCGAGTC
    GAAGTCGTACTTGAAGGTCCCCATGTGGCAGCTCCATTGACCAGGAAGGCAAGCAACCGGAGCCCCCAAGTTAACCAATG
    CTTTTGGGCCAATTCGTTTGACAATTGAGGAAGTGAGCCGCAACTTTTAAATGAAGAAACATGGGGAATGGCATGAACAA
    GGTCTTGCCGGGACTTTATGTGGGCAACTATCGCGACTCCAAGGATCACGCTCAGCTGGAAAGATTCAAGATCTCGCACA
    TCATCGCCATACATGACAGTCCGCGGCGTTTGCTGCCGGACAAGCACTACCTGTGCGTGATGGCCTCGGATACGCCAGAC
    CAGAATCTCTCCCAATACTTTTCCGTCTGCAACGACTTCATACACGCCGCCCGGCTTCGCGAAGGCAACGTACTCATCCA
    CTGCCTGGCGGGGATGTCGCGCTCGGTGACCGTGGCCGTGGCCTATATCATGACGGCCACACACCTGAACTGGAAGGAGG
    CACTTAAGGTGGTTCGAGCTGGTCGCGCTGTAGCCAATCCAAATGCTGGATTCCAGAGCCAACTGCAGGAGTTTGAGCAG
    TTTAAGCTGTCCGAGGAACGCCGCCGCTTGAGGGAACGCTTCCCATCCTCGGCTCTGGAGCAACTGGATCGCACTAAGGT
    GGCCACCGCGCTGGACAACTACCAGGAGTTGCTGCAGAACCGCGACATCTGCGAGGGTAACTGCTCCCGCGGCGAAAAAT
    GTCCTACAGGTGCGGACCAAGACGTAGATGGTGTTAATATTGGTCAGGGGGAGGAAGAAGACGAGGGTGAAGGCGAGGGC
    TTGGGCAAAGTCAGGCGAAGCTTGGCTTACACTCTAAACTTTGCGGTGGACCAATGGAAGAGCAGTACCATTAAAAAGGA
    TCCGAACAATGACCCCCCACCGGATTTTAGCTTGGGCAAGGACCTTATGCCAAGTGTGCGTTACTACGATCCGGAGTGCG
    ACAAGGGTCAATTTGTTAAGAATGTGGATTCGGACAATGATGAAGAACAGTATATTGCTTTCAAGACCTCAACGAAGCTA
    GAAGCCAAGTCCACGTCAAAATTAAAAAAACGACGTGTACCAAAATCGGAATCCTGCAACCAAAGCTGTCTTGAGAAGCC
    ATAGTTACCACAAATAAAATAATCATCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    
    Library Information (1)
    Library: AT_cDNA
    Description
    Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.
    Sample preparation
    Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.
    Protocol
    RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.
    Comment
    Reported As
    Symbol Synonyms
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 2 )
    Crossreferences
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)