FB2025_01 , released February 20, 2025
Clone: Dmel\bs83b09
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General Information
Symbol
Dmel\bs83b09
Species
D. melanogaster
Name
bs83b09
FlyBase ID
FBcl0046925
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG43450
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    BS_cDNA
    • BS cDNA library of D. melanogaster, adult male, testis including ejaculatory duct and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      ejaculatory duct
      (Andrews et al., 2000)
      seminal vesicle
      (Andrews et al., 2000)
      testis
      (Andrews et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      295
      NCBI
      BE978812
      5' sequence dataSequence Downloader
      GCACCAGATCTTAGGAGGACGCCCTGCCCCCAATTAAACACCACCGCAACCACAATGAAGAAATGTGTAATTGCCTGTTC
GTCTTTCGGTTCTCCGTCAACGTCGTGCTGCTCTCATTGGCCACGATTATTCTCATCCTCCGCATGGTGAGCTGATCCAG
GATGGGCGCGGGGATCCCCTTCCAGCCGACAAATTCTTGTTTCTTATTATCTTTTATAAAAAACCCTCACTAAGCGCACA
AATAAATGTTTGTTGAGCTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: BS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from young adult male testes, seminal vesicles, and ejaculatory ducts.

      (Andrews et al., 2000)
      Sample preparation

      Adult male testes were dissected from 1-5 day old y[1] w[67c1] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles.

      Protocol

      Poly(A)+ RNA was purified from dissected testis. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.

      (Andrews et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      BS
      BS_cDNA
      References (1)
      Research paper (1)
      Andrews et al., 2000, Genome Res. 10(12): 2030--2043
      Gene discovery using computational and microarray analysis of transcription in the Drosophila melanogaster testis. [FBrf0132348]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)