FB2025_02 , released April 17, 2025
Clone: Dmel\GH12319
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General Information
Symbol
Dmel\GH12319
Species
D. melanogaster
Name
GH12319
FlyBase ID
FBcl0114747
Feature type
cDNA_clone
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • suspect: probable genomic DNA
Library
GH_cDNA
  • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,366
    NCBI
    AY119504
    Full Length sequence dataSequence Downloader
    GAGAGAGGGAATCAGAAATCAGCGAGGACGAAGCCAGAGGCAGTTGAGCAAGAAGAAGAGGAACAGCAGGAGCAACAGCA
AGCGTAAGGATTTGATCTGTGCATGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTACTTTAAAGAGACGTAAAATTGCGAA
ATGAAATAATGGAAATTTATGTGGCTAGCGAACTTCAAGTGGCCACTTCACTGCCCTCACACCCACATATGTACATACAC
AACCACAAGCACAAACACTCACCCACACATCCATGCAGCACTTTTATTTATTTATTGCGATTTTTGGGTCAAACTAAAGT
TGCAATGATGATGTTGATAATTACCAGTTCGTTCACATCGCCAGCAGTTCAAAAGTCTTTGGGGGAGTTCCCCTTTTCCA
CCCACACGCACACAGAAGCACTCACATAAAATCGCCCACTCGCACACAAGCATACACACGCGCCCTCGAGTTGGGTGAGT
GTGTGTGTGTGCTTTTGTTTTAGTCCTTGGGTCCTTGCGGGGAAAATTACTTTGTTGTTGCTGTTGCCTTTGTTGTCGTT
GTGGCAAGGAACGCGGTCTTTGACAGCTCTCAAGTAAAACGCGCACCTGCGAATTCGCCATAAATTAGGCCCGCGTCCCG
CATATAGCAGGATATATAGAAGGAGATATATTTAGTGCGACGTTGGGCCGATGTCTAAGCACTCAAAGCGCAGGCAGGAT
AAGCCCCATGGAAAAAACGCGCCAAACCTCAAGGCTATTACAGTGGATACTAGGGATAGTGCGATCCAAGCCACCCATTT
GCCAAAGCTCAAACCACTTTTATTTTATTGGCACGAAAGAAAGCTTTAGCTTAAGAACAACGACAGCAAAAAAGCCGAAC
ATTCTTATTAATTTTTAAATAGCACATGTCCTGTTATTGATTTTTCATCCATCTGAAAAACGAAACAAATATTCATTTTA
TTTATGTTTTCAAAACATATTTGATTCATGTGGGGCGGCAGGCTGACTTAAAAGATTTTGTTTTTACTCTCAGAAAACAG
AAATCGTGTTTTTCCATTTATGTTGTTTTGCAAACATGGCTAGCTTATCAAAACCGATATTGTTTACACGGCTCATAGTG
CATCATACATCATCATACAATCATATTTATTTAATACTTCTAAAACAGCATGTGCGTTTTTAGCAACATGGCACAAAATT
CTCGTTGATTTTGGTTTGATTCTGGTGTTTTAATGGTTAGCTTTTATTTGATTAGTTAAAACTGTAAGAACTTTTTACAA
AAAATCAATATGAATATATTTGCTTTTAGCAATCCAATTCTTTAAGTTAATTAAGCCATCCAATCCAGATAGTAGCTCAA
GTTAATAAAAAACGAATTCTCCATGTCGTTGGAAAGTTGTCAATTAGTTTATATTGCTAGTCGCGCAACTTTTAAATGCA
ATTGGATTGAATTTATTCGCTAATTGCTCGAAACTCAACAAAAACGAATGAAATGCTCCATATATGACATTCAGTTATGG
CAATGAATTTAGTATTATTTTTTTATGAATGCCCTATAATTTCTTGGGCTAAAGTTAATGGTCACAAGACTGCGTGCTTT
TAAGTAATACCTTTTTTAAAATAAAAAATAAGCTTGTTAAGAAGGTCGCCTTTATCAGATTTCGTGTTAAAATAAAATTA
CAGAGTAATTTACCTGTACCCCCCGTGCCACCCTTTAAATGAGTCGTACCTTTAGGGCTTCCTCATAAAACCGTCTCAGT
GCGCAGGAATAGCGACGAAGATTAGCCCAATTAGCGTGTCTCCACTGTGGTCGCTCCGCTGTCTCTGGCCTGTCATTTTG
GCCAGTTTCGGGGAAGTCGAGGCTGTGGCATGCGTGAATCAAGTTGATTCGAGTTGCTACCCTTAAGAAGAGCCTTTGTT
TTTTCTGTGTTTTCCATGTATTCTGTGGTTGCTGGCCACCCAATTGTCCCTCCACGGATTTTGTGCTCTATTTACCTGCC
TTATAAAAACACAATTTATTGCCGTACCAACGTTTTACCTTTGTGTTTGGCTGCTGTATTGGAATCGGGTTCTGTTTTCC
GCTCCTGTTTCCATTTCTTTTGGACCCAGCTTTTTATTGTATTTCGCTGTTGCTGTTTTTAGTTTTAGTTTCAGTTTTCG
CTCTGCTTTGTTTTATTTCGTCTCGTTTCATTTCCAACTTAACTTTTCCGCTCTGAGGTTGTGATTCTGATTCCGATTCC
GATTCTGTGCGCTCGGGGACTTCCCCTTTATTGGCTTGCTGTAGAAAGTTTTTGCGTGACATCAGCCATGGATATTATTC
CGCTGGAAGCCATTTTGTTTGGCCTTGTTTGCCTTTTGAATCAATAAACTAGCCATGGCATCGAGAGCAGAGCTCCTTAA
TAATTGAAAAGCAGCGGGAGCTTAGGTTTATGCCCTGCCCTCTTTTTTTTGCTTTAAGCCACAAAAGGACACTTGGTCGG
AACGCTCAAAAGCAGGCAACACTTTATGTGCATGCAAATAATTCGTTTACCACTTATCCGGCTCACTTTCACTGCAAACG
CCATAGTTTTCGGCCATATCTGTGGACAAAAGTTTGCGACTCTCCAGAGCAATTTGATCATTTGTTATGGTCTGGTTGAA
ACTCTGGTATTCCCACAGACGAAAAATTCCGCAACACTTGGTGGTCACGTAGTATCTAGTGGGCGAAAGTTTTGAATTTA
CGATTTACGAGGCATAAACTTTATGCCACAGCTTTCACTCGATTCGAATGAAAGTCGTCGGTAATCTGTGATAACGAATA
TGGATTCCGTTAAGCAGCCAGCCAACTATCGGCTATCAGATATCAGAAATCGAGGATGAAAGGCCGCTGCCCAATGAGCT
TTTAATTGTGTAATTAAATCACTGCCACTTGGAGCACTCATCTTTGTTCATTAGGCTAATTGGGTGGATCAACTTATTCT
CAAAACGAGAAAGTTAACAATGGATATTGACGATTGTCGAGAACGTTGGGCGCCACTCGTTCTCGAACGATTGCCCATTA
TGAACTTCGATACCAATGCGTTCTAATTAGTTCCTGAGGTCGCATTTTCCAGGCAAATGAGCTCACGTTTTTGTGCCGCC
GCTGCTTACCTGAATAATGAGTTTTCCATCCACACATCAACGGCATGCATTTCGAGCTCTCGAATGGTAAAAACGGAGCA
CGGAAAGCGGGGAATACTCCGTACCGGCACCCACACAAAAGTGAATTCTGGGCGACGGGCGTCCACATTCTACATTGCTG
GCCATTCGGAGGCGTTGTACCGGAAAGAAATGCCATTAAAAACGCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAA
    Library Information (1)
    Library: GH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Rubin et al., 2000)
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    (Rubin et al., 2000)
    Comment

    The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

    (Rubin et al., 2000)
    Reported As
    Symbol Synonyms
    GH
    (Rubin et al., 2000)
    GH_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)