Clone Dmel\GH15984

General Information
Symbol	Dmel\GH15984	Species	D. melanogaster
Name	GH15984	FlyBase ID	FBcl0126443
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\RhoGAP1A

Collection Status	BDGP
Known Problems	none
Library	GH
Strain	y; cn bw sp
Tissue Source	adult stage head
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	5068
GenBank	BT024449
Full Length sequence data	TGGAAATCTGCGAAAATATTTCTGTTTTGTCTAATTGTTAAAGTCCATTC AATGTGAAAATAAATTGTAGGAATGGTGAATAAAACCCGTGTTCGAAAAT GAAACGGGAAAAAGGCGACAACGCACAGCTGGTGCTTGTTCTTATATAAA AGGCAACAACGGCCTCTCCGCGTCATCATCTTGAATAATAATCATCAGCA TTTTTCAATTAATAAGGAAAACGGTTAATATAAAAGCAAGCACCCGAAGA AGGAGCTTGCACCAACATGAGCGTTTTCGATGACTTCCAGCGTCTGTGGA TGCAGCGATTTCCGCAAAGCTCTCTATCCGATGCATGGGAACAAGATGTT CGGGCTAGTCTCGAGCGACACAAAGTGCGCATTAATGAGCTAAGCAAGGA GCTTGAGCAGGAGACGCTCTATGTGGAGTATCTAGAGCGTCTGCTGTCCG ACGTGGAACGGTATCGCGTTTCTGGCGGGGATCCAAAGGCACTCTTTGAG GCTGCTAGCGGTAGTTGTGCTAGCAGCAATAACAGCAACTGCAGCAGCAC CACCAATGGAGCTACACAAGAAGAAAGCAACACTAATCTGGACAAAGATT TTAAATCTAGTTTAAATCTGCGCGAGTCTCCTTCTTCCACTGGCGCCAAG GACAAGGATCGCCTGTCGCTCAACCCAGACAACAAATACACAACACACAC AAATGCCGTTATAGGTGGGATCGGATCGGAAAAGGTGAGCGGTCCACTTC ACTCCACTCAGATCACGGCGGACGGCAAACGAACAGGGGAGCTGTATTTA AAGCGGGAAGAAGACAAATGCAAGGATAGTAATAGTGAGGACACCGATGG GGCCCTGCAGCAGTGCATCACGGAATTGGCGGCGTCTTTAAAAGCCCAGC CGGATAACGTGGCAGGAGGGCGTCTAGAAAACGATGTCGATATCGATAAA CCGCTGCGCAAGTCGACGTCACAATACGTTACTGTCATTCAGGTCAAAGA GGCCAAAGACAAAGAAAGCGCTGATGGAACTACCTCGTCATCGGGGATCG GTGAACCGCAGCAGCAACCCACTCCACCATCCACATTTTCTCGATATTCG GAGGCCACCGCTCCACCGCTTTCGCCATCGCTCACATCGTACGCTGCCCA CGTAGAGGCCAAGAAGAAAATGCCACCCAGACCTCCGCCAAAGAATATTC GGCGAGTTGAGCCCCCTACCATTCCCAGCCAGCAGCTATCGTCTTCGCCC AAAAGGGAGACATATATGATCAACACGATGCCAAGCAGTTCAAGCGGCAG TCTCTGCATTGACAGCCTGGGCAATTCGCTGGAGCGGAACATCAAGCCGT CTGATATACTACGTCAGAAATCATCTGATAATTTGGATTCCAAGTACTCG GCAAAACGGAAAACCACACCGGAGCTTAGTAAATTTGTGAACGTAAACAA TCCTGAATTAATGGAGGAGCTCGGTCGTAAGATGGTAAGCAAGGCAGACA GCCTGGAACAAACGAGGAGGAACGACTCGTCGCCTTCTGGCTGTGGTAGC CTAGGACGCACTTCCTCCACGCCTGGGCGATCGTTGACTCCTGGACGAAC CGGAGTTGGTGCCTCGCCAACTGGTAGTCTAAGTAAAACCGGAAAACTGG CAATGGCGGACAGCAGCTCCACTAGCAGTCTAGAGAGGAGATCGTGCCTG CAGGCTGTCGATGCGGACATTGGAACGGAATCGCGGGAATCGGGTGCCAA CCAGCGTAGTGTAGGGTCCAAGGAGTCGCTGACATCACAAGGCATATCGG AGGTGATCAAGAGCTACGAAAGCGTGTCTTCGCTAAGTTCGGAAAGTGCA AAGGCGGTGGCTTCCCAACAGTCGGCGGCAGCGGGCGGAGCTTTAGCGGA GTGTGAACCGTACTATGATAGCGTACCGCTCGACAACGGCGACGGAGAAT ACGTTTTTATTAAGCCCGGCCGCACCGGATCCTCATCCAGTCGCGATGAT TTGTCGACGCCTGGGAGCAGCATTTTGCCTTTGCCTAATGTAGCGGATCC CGAATCTCCAGGCAGGACCTCTAACTACGTCAACATCGATTTCTTCCTTC AGCAAGGAATTGAGACGCGCTCCAGTTCGCTAGACAGTGACGGGGAGTAC GAGGGTCCGCCCATATTACGCACCATTTCGCACGACGATCAAACCACAAC ACAAACTACTCCGGGAGCAATTCGCAAGAACTTAGTTTCGGCGATACTTG TTTGTGGAAATGCGCGCGGTGCTAAAATACGTTCCATACTTAGCTCAATT ATTCAAAGTGAGACTATCTACGTGGAATGCCTCAATAAAATGATGCAGTA CAAGAAGGCCATCCACGCAACCCTAACTACTTCGCAGCCTGTGATCAGGG AGGAGGAGGAGAACACAATTTTCTTTAAAATTGATGAGCTTTACGATCTG CATACCGGTTTCTTATCTGATTTAAAGACTATTGTGTCTCACGAGGGTGG TGATGTGCTGATCGGTGAGCCGTTTCGTCGACTGGCGGAGATGTTTGACT TATACAGTGCCTTTTTGCACAACTACAAAAATGCTATTGAGACTGTGAAG AAGTGCAGTGCCAATAACCCCCAGTTTAAAAAGATCGTCTCCACCATTGT GCTTAATCTGCAGACGGAACAATCGCTAACTCTCGAGGATCTATTGCACA AACCTGTTGCGAGGGTACAAATCAACGCATTGGTCTTCAACGATTTGCTA CGCGAAACCCCAAATGCGCATCCGGATCACCAGCCATTAAGGCAGGCGCA AAAGATTATTCAGATGTTCCTCAATCAGTTCAATGTGGTCAATCAGCGGT TGCCCATTGAATCGAATCGAAATTTAAGACGCATGGTGCGTAATTCTTTT ATTGTAGAACTTGTCGATGGACATCGTAAGCTGCGGCATCTCTTTCTTTT TAATGATGTCATCGCATGTGCCAAATACAAAGCATTAGGCCGCGATCGTA TCGACTATGAGCTCAAATGGTTCATTCCGCTAAAAGACGTATCCATTTAC GAGGAAGCAGATCCGGCGGTCGAGCTTAAGGAATCTAGTCCAGCAAACAT TTCGCAAGTAAAACGCAATTTGCGATCCGTTCGAGATCAATTGGCCATGG AGGTGGCTAACAGTGGAAGTGGAGCCTTCCGTTCCGGTGATAAGTATCGC CGCAAGCTGGCGGATCTGGAATCACAGCTGGTGCTGGCAACGCCCAATTT AGTGTTGCGCCTGGGGAACAAGGCAAATAATAAAACCATAACCTTCTTTC TTAGCTCGGATTTTGAGAGGACGCAGTGGATCGACTCAATCTTATCCCTT AAGCAAAAATGCAACCTGCCTGGAGCAAATACTATTAATTCCCTGGAGGT TACCGCCTTCATTGTAGCCATGCAGAAGGGCATGAAAACCGAAATGGGTT CATATCTTATGCGTAACACTAACGACGAGAGCCTGTTGGTTGGGGACCTG TACATGGGAGTTCATGGATTGGAAGGACTGGAGCAAGCGAACGACCTGTA CATCTGCGTCGAGGTGGACTCCTATGGCCACTATTTTCGCAAGGCCACCA CCAAGAAGATATGTCGCAGTCAAACGCCGCTCTGGAACGAAAGCTTTATG CTGGAACTGGAGGGCAGCCAGAATGTACGCATTCTTCTTTATGAGGCTAA GGAGCGACCGCTTCTTAAAGCCAAGCACATACTTAAGTTGAGTTTAAGTT GGCTGACAGAAACAACGCAGCCGAAGTCAATTAAACTTACTGAGACCTTA GAGCTCGGCTGTAGCTTCCGTTTTATACCAGGCGAGCTCTTCCGCGGCAG CACAAAGCCAGGAGCCCTCTTCGGTGCCAAAATGAGTCAAGTATTAAAAC GTGAAAAACGCGACATTCCGTTCATCATAGGCGCCTGCATTCGGGAGGTA GAGCGGCGTGGGATGCTGGAGGTTGGCTGCTACCGCGTCAGCGGTTCCGC TTCTGATTTGGCGAAGCTGAAGAAGGCCTTTGAGTCTGATGCCTATGAGG CGGAGCAGTTGCTACGCGAGGTGGACATCCATTCGGTCACTGGCATTCTG AAAACTTTTTTAAGGGAGCTCCCCGAGGCCCTTTTCACGGATCAACTTTA TCCACGATTTTTCGACACATTCAGCGCCTTTAGCAACAACAACGAATCCA CACGAATCAACGAGCTTCTAAAAGTTTTTGAGGAGTTGCCACAGGCCAAC AAAGCCTCCATTACTTCGATATTAGATCATTTAATAAGAGTCCACGAAAA GGAGACAGATAACAAGATGTCGTTGCACAATCTGGCCATGGTGTTTGGAC CAACCTTGCTAAGGCCGGGCCAAACGCAGGTGAAGCAGAAGGATCCACTG GCAGCCAGCACAGTCGACGTTATGGCTCAGGCAGGAATCCTATACTGCTT TCTTCAGGCACGGATCAAGAAGGATTAGGATTTCGTGAGCAGTTTACACA GATACATATGGACATATATATATGTACAAAAATTATATTTTTTACAAACA ATAGCAATAATACGATGGAAGTTGATGGACAGTCTACTCATACGTCACGT CTCTATCTTAATGACAACCCCTTATATTATATAACAAACCGCCCAACTAC CTTGAAACTACGGAAAAGGCCATTAAATTCCGAAAACACCATAAAACACT GGTGCTGCCTACTGTATATACCAGAATCCGCAACTGCAATTATAAACCGA ATATGATGATATGACGTGGAAAAGGGAAACACCAGGATCTATGTAAGGAT CCAGGGATGTCACATATCAGCTCAATGTCGGGTCATCCATGTACATCGCA CACCTGGAACTAGCAAGTGTTTAACACTTGTACATCGCTGCTTCTCCGTT ACTGAAAATTACTCCCATATATTACCCGTACTTTACTAGACATGTACATT TGGATGCAAGTTCTGCTTACCGTAACAAGTGCAAAAAAGAGCAACAGAAA AATTGCAAATATTTTCAACAATTAAAATATATATCTACAAACATAAAAAA AAAAAAAAAAAAAAAAAA
Library Information
Comments	The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, we chose to remake the head library. mRNA source--adult heads, from an isogenic y; cn bw sp strain, polyA+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
Library Synonyms
Reported As
Symbol Synonym	GH (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0126443
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]