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General Information
Symbol
Dmel\GH02419
Species
D. melanogaster
Name
GH02419
FlyBase ID
FBcl0136315
Feature type
Computed gene(s)
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,505
      NCBI
      Full Length sequence dataSequence Downloader
      AGAGGCCGATGCGATCGTTTGGGTCGGTTGGAACTTTCTCTATAAGTCGGTTGTTCCCGCAACCTCTGTGTGCCTGTGTG
      TGTATATACATATATATATATCGTGCAGTACTGTGCAAGTGAAAAATCAAAAGTTGAATGTAAAGTGCACAAACGTAAAT
      AAATAAAATACGAAAAGTGCAATGTCTGCGAAGACTTCGGTGTGGCTGGTGTTCTTTTGCGCCCTCGGCTGCCTGATTCA
      CGATGCCGATTTGCGGAGTATTCAACCAAAAGCATCCCAGTGCGGCTATCAGAGCTGCCATCCAACCAAACCCAACATGT
      TGAACGTGCACCTGGTGGCCCACACACATGACGATGTGGGATGGCTGAAGACCGTTGACCAATACTACTATGGCAGCGAG
      ACCAAGATCCAAAAGGCTGGAGTCCAGTACATAATCGATTCGGTTGTGGAGGCTCTGCTCAGGGATCCCGAGAAGCGTTT
      CATCTATGTGGAATCTGCCTTCTTCTTCAAGTGGTGGAAGGAGCAGAAGCCCAAGGTCCAGGAGGCAGTAAAGATGCTGG
      TGGAGCAGGGAAGACTCGAATTTATTGGCGGCGCCTGGAGCATGAATGACGAGGCCACCACCCATTACCAGAGTGTGATT
      GATCAGTTCTCCTGGGGATTGAGACTTTTGAATGATACCTTTGGTGAGTGCGGACGTCCGCGTGTTGGATGGCAAATAGA
      TCCCTTTGGTCATTCCCGTGAAATGGCATCCATGTTTGCGCAAATGGGTTTCGATGGCATGTTCTTCGGTAGACTGGACT
      ACCAGGATAAGGACGAGCGTCTGATGACCAAAAATGCAGAGATGATATGGCATGGATCAGCGAATTTGGGAGAGGAAGCG
      GATCTGTTCAGTGGCGCCCTCTACAACAACTACCAGGCACCAGATGGCTTCTGCTTCGATATCCTATGCAACGATGCACC
      CATAATCGATGGCAAACACAGTCCCGACAATAATGTTAAAGAGCGCATCGATACGTTTTTGGATTTCGCCAAGACCCAAT
      CGCAATACTACCGCACCAACAATATCATTGTCACAATGGGCGGTGATTTTACATACCAGGCTGCTCAAGTTTACTATAAG
      AACCTCGACAAGTTGATACGATATGGCAATGAACGCCAGGCAAATGGTTCGAATATCAATCTTCTATACTCCACTCCATC
      GTGCTATCTGAAATCCCTGCACGATGCCGGTATCACCTGGCCAACCAAGAGCGACGACTTCTTCCCATACGCTTCCGATC
      CTCATGCTTACTGGACGGGATATTTCACATCGCGACCCACCTTGAAGCGATTCGAACGGGATGGCAATCATTTCCTGCAG
      GTCTGCAAGCAGTTGAGTGCCCTGGCGCCCAAGAAACCCGAAGAATTCGATCCCCACTTGACCTTCATGAGGGAAACGCT
      CGGAATAATGCAGCATCACGATGCCATAACTGGAACAGAGAAGGAAAAGGTGGCTCTGGACTATGCCAAGCGGATGAGTG
      TTGCCTTCAGAGCTTGTGGAGCTACCACTCGTAATGCCTTGAATCAATTGACGGTGCAATCGAAGGATAATGTCAAGGAT
      ACGTCGGCCAAATATGTGTTTGAGTTCAAAACGTGTGCCCTACTAAATATCACCTCGTGTCCCGTGTCCGAGGCGAACGA
      TAGATTTGCTCTAACCCTCTACAATCCACTGGCTCACACGGTTAATGAGTATGTGAGGATTCCTGTGCCATATTCCAACT
      ACAGGATCATCGATAACAAGGGTGTTACTCTGGAAAGCCAAGCTGTTCCGATTCCCCAAGTGCTGATCGACATTAAGCAC
      AGAAATTCCACAGCCAAATACGAAATTGTTTTCCTAGCCACCAATATTCCAGCTTTGGGATACCGTACATACTATGTTGA
      AAAACTGGATAGTACCGAGGGTAATACTAGATCAAAGGCTTTGCCGAAAAGAACCTCCAGCGTTACAGTGATTGGAAATA
      GTCACATTCAACTTGGTTTCGACACCAATGGTTTCCTTTCCGAAGTCACTGCTGATGGCCTAACCAGATTGGTCAGTCAG
      GAGTTCCTATTCTATGAGGGTGCTGTTGGAAACAACGCGGAGTTCCTGAATCGATCATCTGGTGCCTATATCTTCCGACC
      CAACGAGAACAAGATTCACTTTGCCACCGATCAAGTCGAAATTGAAGTCTACAAGGGTGATCTGGTTCATGAGGTGCATC
      AGAAGTTCAACGATTGGATTAGCCAGGTGGTGCGTGTTTACAACAAGGATTCATACGCCGAATTCGAATGGTTGGTTGGA
      CCCATTCCCATTGATGATGGCATTGGCAAGGAGGTGATTACTCGCTTCAACTCTGACATCGCTTCGGATGGCATTTTCCG
      CACCGATTCAAATGGTCGCGAGATGATCAAGAGGAAAATCAACCATCGCGATACGTGGAGTGTGAAAATCAACGAAGCCG
      TTGCTGGCAATTACTATCCCATTACGACTAAAATAGATGTGGAGGATGACACAGCCCGCATGGCCATACTTACGGATAGA
      GCCCAGGGTGGTAGCTCCTTGAAAGACGGATCCTTGGAACTGATGGTTCACCGTCGTCTGTTGAAGGACGATGCCTTTGG
      TGTGGGCGAGGCCTTGAATGAAACCGAGTATGGCGATGGACTGATTGCCCGAGGCAAGCATCATCTGTTCTTCGGCAAGT
      CGACGGATCGGGAAGGTGTTTCCCTCAAGGGAATCGAGAGGCTAACCCAGCTGGAGAAACTGCTGCCCACATGGAAGTTC
      TTTAGCAATATGGAGGATTACAGTGCCGATGAATGGCAGACAGCTTTCACCAATATCTTTAGTGGAATTTCATTGGTTCT
      GCCGAAGCCCGTGCATCTGCTCACCTTGGAGCCCTGGCATGAGAACCAACTCCTTGTGCGCTTCGAGCACATCATGGAGA
      ACGGCGAGGATGCATCCTACTCCCAGCCCGTGCAGTTCAATGTCAAGAACGTGCTGAGTGCCTTCGATGTCGAGGGCATC
      CGTGAAACGACTCTGGATGGCAATGCCTGGCTGGACGAAAGTCGACGACTTCAGTTTGCGCCGGATCCTGAAGAAGCCGC
      GTTCAACACGTATGCCACATTCTCGCAACCCGCCGAATCCGTTCACCTACTAAGTGCCGAGAAGCCCATGTTGGGTGTTA
      AGTACGCCGACGAAGCCTTGCCAGCTGGACAGCTGGGTGCCGAGAGCAATCGCATTAGGCGCGAAACAGAAACGCGACAG
      GAGAAAAAGGACGAGGGACGCAGCTCCAAGTCGACTGAGGGTCCTTACAACTCCTTCAAGTCAGATAGTAGCAACCAGGA
      ATACATTATCGAGCTGAGCCCAATGGAAATCAGAACATTCATTGTATACCTAACACCAGCGTAAAATACTTTTTTTTACT
      CTAAAGCAACGAAAGTATATGCTTAATAAAATTTAATGAGTAATATAAAAAAAAAAAAAAAAAAA
      
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      Reported As
      Symbol Synonyms
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)