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General Information
Symbol
Dmel\GM04727
Species
D. melanogaster
Name
GM04727
FlyBase ID
FBcl0140591
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI04117
Library
GM_pBS_cDNA
  • GM cDNA library of D. melanogaster, Oregon-R strain, adult female, ovary, pBS vector.
Strain
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    1,250
    NCBI
    Full Length sequence dataSequence Downloader
    ACGATGTTCCCGCAACGCAGCTCGGAGCTGTACCGCACTCCGGTGACCCACCAGCCTCCGCCGGCACTGGAGCTGGCCGG
    GAATCTGGAGTACCCGAACCTCAACATTGCGGACCTAAACGCGCGATTGGAAAACCTTTCGCCGCGACTGCACGACTGCT
    GGGACAGCATCGCTATCAATGATCACAACCACCTGGCCCTGGCCACAAACCGACGCGAAGGACGCCAATGGTGGGGCATG
    TTGTTCGGCTACGGACGCGACCAGATGCACCACATGAGCGTGGACTCGGCGAACTTCAAGCTGCAGGCGGAGCACACGGT
    GAACATTGTGCGCTACGCTGAGGATGATTTCCTACTGGTGGCCCTTGGGGATACCCGACTCCAGGCCTGGTCCACATACT
    CCAAGGTGCGAGATTCTCAGTCGCCATACTGCCTCTTCCTCGTGGGCGAGTCCAGTGCGCATCCCACACCCATCAGCCAA
    CTGAGCGTCTTCAAGGCGGATCCCCGCACTGCCGTTTCGGGCTCAGCGGACAGTACCATCAATGTCTGGGATCTCTCGGG
    CGCCGATATGGTGAGCACCTACAGATCGCGCTCCTCGCATACCGACAAGTTGACTGGCCTGGCCACACCCGCAGCTTCAG
    TCGATAAATTTGTGACCTGTGATCGTGGCGGCTGTGCCCGCCTTTGGGATGTCCGGGCAGCGGCTCCATCTTCCACGTGC
    CTCTATGCGGATGCCAGCCACGTGCTGAGCTTCACCAGTGCCGCCTGGGCAGCTGCCTCGGAGCTGCAGGGTGACAATCA
    CATCTATCTCGGGGACTACGACGGCAAAGTTCATACTCTGGACATTCGAGTGCCACGAAAACTGGCGGAAACCAGGGAAT
    ACTTCGACAAGGGCCATGTCGCCCAGTTATTAATCAATGGGCCCCATCTGGCTGCGATGAGCAATTTGCCCGCCTCCGTG
    AAAGTGGCCAATGTGCAGGCGGGCCATGAGTTCATCTACACGCACCAGGACACCCATTCTCGCCTAACTGATGCCGTTTG
    GACCGATGATAGCACCCTCATCACCATTGGACATGGCCGCAAAATGGTGACGCACGCGATCAAATAATTTTGCTGGCGAA
    CAAATTCAATTGCTTTATTTCAAAAAGCGGAATGGTTTGCTGAATAGTCATTCTTACATTTAACACTTATAGTTACTGAA
    CAGTTACAAGTAATAAATCGTAATAATAGTCCAAAAAAAAAAAAAAAAAA
    
    Library Information (1)
    Library: GM_pBS_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.

    Sample preparation

    Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.

    Protocol

    RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    Comment
    Reported As
    Symbol Synonyms
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Crossreferences
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)