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General Information
Symbol
Dmel\HL03201
Species
D. melanogaster
Name
HL03201
FlyBase ID
FBcl0149752
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pBS_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pBS vector.
    Strain
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      5' Sequence
      Total bases
      444
      NCBI
      5' sequence dataSequence Downloader
      AGCTCTGTCCAGATGAGGTCAGCGAAAAAACGGATGAAAATAATGAGCCGAACTTGGAGACGAACATGGATGAGAACAAA
      TCGGAACCGGTGGCTCAGCAGGAGGAGTGCTTGAGGGATCTACCGGCGGCACAGAGCAGTTGCCCGAAAATAAGACGCCA
      ACCACAAGCAGCCCGTCGACTGCCGCCTGGCCAGCGCGTGGGGATAGCAACGCTATTCCACGACTTTCGCGTCAGTATCT
      TGGGGCTTACACTTCGACTGGCAGTGGCTCCACTAGTGGAGGTTCCGCGCCAACTTCCGCCTTGACGACAACGGCGGGTA
      GTGGGGCTAACAGCGAAACAGAAAGCAGCGCCCAGGAAACAACCGGCGAGACGACCATTAACGGACTGACAAACAGCCTG
      GACCAGATGGAGATAACGGCCAAAAAGAAGTGCCGCAGGTGATA
      
      Library Information (1)
      Library: HL_pBS_cDNA
      Description
      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
      Sample preparation
      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
      Protocol
      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL01001-HL07796, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
      Comment
      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
      Reported As
      Symbol Synonyms
      HL_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)