FB2025_02 , released April 17, 2025
Clone: Dmel\HL08052
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General Information
Symbol
Dmel\HL08052
Species
D. melanogaster
Name
HL08052
FlyBase ID
FBcl0150821
Feature type
cDNA_clone
Computed gene(s)
Dmel\mal
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pOT2_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      2,738
      NCBI
      AY118319
      Full Length sequence dataSequence Downloader
      CAGCGAAGAAGAGTATGGCGCAACAAATGCAAGCCGGTCGCAAATAAATAAAGCCCGCTTTGCAGCCAACACGAATAGTT
AGTTCCCAACATGACATCATATCGGCCGGAGTTCTCCGCATCGGAGCAGTCGCAAATAGACGCGGAATTCTCTAGATTGG
CCAGCAACAAGAGTGTTTACCTGGATCATGCGGGCACCACTCTCTATGCCGAGAGCCAGGTGACGGCCGCCGCGGAGCAA
CTGCAGCGCAATGTCATCTGCAATCCGCACACCTGCCGCCTCACCGGCGACTTCGTCGACCAGGTGCGATTCAAAATACT
GGAATTCTTTAACACCACCGCCGAAGATTACCATGTGATATTCACGGCGAATGCCACCGCTGCTTTGTCCCTGGTGGCGG
AAAACTTTGACTTTGGATCCTCTGGTGAGTTTCACTTCTGCCAGGAGAACCACACCTCAGTGCTGGGAATGCGGGAAAGG
GTCCGGGAAAACGGTATATACATGCTGAGGGAGAATGAAATCTCTGGCGGAAAGCACAAGGCGAACGGGAAAGTTCACGA
GGTGTCCGGAAAAACAGGAAACTCCTTGCTGACCTTTTCGGCCCAATGCAACTTTAGTGGTTACAAAATCCCACTGGAAG
TCATTGAGCAAATCCAAATCGATGGCTTGGCCAAGCCGGGAAAAGAGCTTTGGAGCTCTTTGGGAGAGAAGAAGAAGAAT
ATGCACAACGATTACTATATCTGCCTGGATGCGGCGTCTTTTGTGGCCACCAGTCCGTTGGATCTGCAAAAGTATAGACC
AGATTACGTGTGCCTTAGTTTCTACAAGATCTTCGGTTATCCCACGGGCGTGGGAGCCCTGCTCGTGAGTCGACGAGGAG
CCGAGGTCTTCCAGAAGCGCCGCTTTTTTGGCGGTGGTACCATCAACTACGCATATCCGCATGCCATGGATTACCAGCTG
AGAGAGACCTTCCATCAGCGCTACGAGGACGGCACCCTGCCATTCCTCTCCATTGTCGGGTTGCTCGAGGGCTTTCGAAC
GCTGGAGAGATTGGTGCCCAGAACGGACGAGTTTAGCACCATGGAGAGGATTTCCAGACACGTCTTTGGTCTGGCCAAGT
ATCTGGAGGATCAGCTGCGCCAGCTGCATCATCCCAATGGTGAGCCCCTCGTGAAGCTGTACAATAAAGTGGGCTACCAA
GACAAATCCCGGCAGGGTGGCATCGTGGCCTTTAACGTGCGCACTGAATCGGGTTCGTTTGTGGGATTCGGCGAGATCGC
CTGTGTGGCTGCTCTGCACGGAATACTCCTGCGGACTGGTTGCTTCTGCAACATCGGAGCCTGCCAATATTACCTAGGCC
TCGATGAAGATGCTTTGGATGCGATTTACAAGCGAGCAGGTCGCATATGCGGCGACTACTTTGATCTGATCGATGGACAG
CCGACGGGAGCGGTACGGGTATCTTTTGGCTATATGACAACTATTCAGGACGTAGACAAGCTGCTCCAGATGCTGCGCTC
CAGTTATTTGGCAACCAAGCCGCTGCAGCGGATTCAGTTCATCGAGGAGCAGGCGGAGCAATTGCCGCCGTTGCTGAAGG
AGCGAGTCCAGCTCTTGAGGCCAAAACTATTGCAGATGGCCATATATCCGGTAAAGTCTTGTGCCGCCTTTAAGATCGAA
TTACCGGGCTCCTGGCCATTGACGGATCAAGGTTTGAAATATGACCGTGAGTGGATGATCGTGGACATGAATGGCATGGC
GTTGACCCAGAAACGCTGCACGGAGTTGTGCCTAATTAGGCCAGTGATTAAGGTTGACCAATTGGAGCTGCAGTTTGGAG
AAAATTCCACCATCTCGGTGCCCCTTTCTCTGGATGACCAGGCAGCCGACACAGCAAAATGCGTAAGCAAGGTGTGCCGA
CAGCCCGTGGAAGGTTTGGATTGCGGCGATAGGGTTGCCCAGTGGTTAAGCGAAAACCTCGGCATGGAGGGCCTCCGTTT
GCTTCGACAATCGGGCCAGAGAAACTCTTCCAAGGATCAGCAGAAGCTAAGTCTGGTTAATCAGGCCCAGTTCTTGCTGC
TAAACAAGTCTTCCGTGCGATCGCTTCAATTTGAAGAGCCCCTCGACGAGACTGTGGATCGTTTTCGGGCAAATATCATC
ATCGACACGGGCAGTGCCTTTGAGGAGCTTACCTACAAGGCCCTGTCCATTGGGGGAATCCAATTTCAGGTGGAGGGTCC
CTGCCAGCGCTGCGACATGATCTGCATTAACCAAAGGACAGGCGAACGGTCGCCAGAAACCTTGACCACCATATCCCGCC
TGCAGAAGGGTCGCATGCGATTCGGCATCTACATCACTAGGATCCCCCAGGACACAAAAGAACTGGAACCCAAGGAGCAA
CACATGACCTGCGGCGATGTTGTCCTTGTGGAATAAGCTCACTTTACATCACTTCAGCGTGGTTTTCTTCGTTCTTTGGT
TCGTTGACCCGAAATGTTTCTATTGTACATAGTTTTAGCACAATATTCACTAAGTAATTCTCTATTACGTTGCATCACGT
TTCGCACTTAGTTTGTTTTTTAAGTACTTCTGAAATAGGAATACGCAAACATTCTTAAAGAAACATTCAGGATAGCTTAA
GTTCAAAGTAACCGGATACCTCTTGCCAGTGTCTTAAGCCTTTAAAAGGCCTTAATTGCAAAATAAACACATTGAAATCG
AAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: HL_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL07801 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      HL_pOT2
      (Rubin et al., 2000)
      HL_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)