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General Information
Symbol
Dmel\HL08079
Species
D. melanogaster
Name
HL08079
FlyBase ID
FBcl0150856
Feature type
Computed gene(s)
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    HL_pOT2_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,529
      NCBI
      Full Length sequence dataSequence Downloader
      GATCGTTCGCCATCGGTTTTATTGTACTTATCAGTGGAGAAACCCGAGAATCTTGTGAAAATGTACCAGCGGCAGGTCTC
      CTTTGATAAGCCAACGCGCGTAGAGGATTCGGCTTACATAGTCGAGGGTGTAGATATTAAGGAGGCCAGGAACACCTGTC
      TTCTGTTTTCGCCCAAGGATTCGTCGTTGTCCAGCGGAGCTTTGGCCAACATTTTGGCCATCTTCAAGAAGCATGACATA
      AACCTGGTGCATATTGAGTCGCGTTCCTCGCTGCGTGTTCCCGGTTATGAGTTTTTCGTGGAGGCCGATGGAAAATCGGG
      AGCCTTGGGAAAAGCCATCGAGGATGTGAAGGAGCAGTGTAGCTACTTTAACATCATTTCGCGTGACTACAAGGATAATG
      CCACGGCTGTGCCCTGGTTCCCGCGGCGTATACGCGATCTGGATCGTTTCGCCAATCAGATTCTGAGCTACGGATCGGAG
      CTGGATGCCGATCATCCTGGTTTTACCGATCCGGAATACCGCAAGCGTCGCAAGTACTTCGCCGACATTGCCTACAACTA
      CAAGCATGGCGAGCCCTTGCCCCATGTGGATTACACCAAGGAGGAGATCGAGACCTGGGGCATTATCTTCAGGAATCTTA
      CGAAGCTGTATAAGACACATGCCTGTCGCGAGTACAACCACGTCTTTCCCTTGCTGGTGGACAACTGTGGATTCCGAGAG
      GATAACATTCCCCAGCTGGAGGATGTGTCCAACTTTTTGAGGGATTGCACAGGTTTCACTCTGCGTCCAGTGGCTGGACT
      ACTCAGCTCTCGGGATTTCCTGGCTGGACTGGCCTTCCGCGTCTTCCACTCCACTCAGTATATCCGACATCCCAGCAAGC
      CCATGTACACACCGGAACCAGATGTCTGCCACGAGCTGATGGGCCACGTGCCGCTTTTTGCAGATCCAGCTTTTGCCCAA
      TTCAGTCAGGAGATCGGGCTAGCTTCCCTGGGAGCACCCGATGATTACATTGAGAAATTGTCCACCATCTTCTGGTTCAC
      TGTTGAGTACGGCGTGTGCCGCCAGGAGGGTGAGTTGAAGGCGTACGGAGCTGGTCTGCTGTCCTCCTACGGGGAACTGG
      AGTATTGCCTCACGGACAAGCCGCAGCTGAAGGACTTTGAGCCGGAGGTCACTGGCGTCACCAAGTATCCCATTACTCAG
      TTCCAGCCTCTGTACTATGTGGCCGATAGTTTCGAAACCGCCAAGGAGAAGACCATTAAATTTGCGAACTCGATTCCCCG
      ACCCTTTGGTGTTCGCTACAATGCCTATACTCAGAGTGTTGAGGTTCTCGACTCGAAGCCCCAGATTTCGAATCTGATGG
      ACAACATTAACTCCGAGTTCCAGATTCTTCAGAATGCCGTTGCCAAGCTGCGCGTCTGAGTAGAATTCATATACATTTTT
      TACTCCCTCTCTTTACTTACTATTAAGAATGAATCAAATAATAAATAACATGCAATTATGTAAGTTTTTGTAAAAAAAAA
      AAAAAAAAA
      
      Library Information (1)
      Library: HL_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL07801 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      Reported As
      Symbol Synonyms
      HL_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)