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General Information
Symbol
Dmel\HL08132
Species
D. melanogaster
Name
HL08132
FlyBase ID
FBcl0150979
Feature type
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    HL_pOT2_cDNA
    • HL cDNA library of D. melanogaster, iso-1 strain, adult head, pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      5' Sequence
      Total bases
      647
      NCBI
      5' sequence dataSequence Downloader
      ACATCAGATCACAAATTGCTGGGAGGTTTGCACCCCAATCAAAGCAAGTTTTACAACAAAAGTCAATTTTCGTTCGACTC
      GATTTCTTCTCAGTGCGTTAAGTTGCAACCATTAAGTGTGAAAATGCCATCCAATATGAATAATAAACTCGACCCAGTGT
      TTAGTAGTGGCTTCGTTAGAAGAGAATTTAATGACAGCGGAATTGCCGACGGCAAGCTCCGCACCATCTCGACTTCGTCT
      TGCGCCACGACCATGTCCTCGGAGTCCTACCGCATCTCTCTCGGCGTAGGGCCTCTGTGGTGGTCCTGATGTCCCTGTCC
      ACCACAGAACAGATCACGATAGGGCATCGCTCTTGACGGGCTACAGCGTAAGTCTTCGGTCGACTCGGCTGGTGGCAGCC
      TGTACGAGGCCAGCTCCCGGGCGTCGTCGTTGTCGTCCTCCCAGTCGGATTGCTCCGACTTGGAGTCGCAGCCCGACATC
      CACTCGCTGTGCTCGGACGATGACTGCCAGGAAGTGCTGCGCCAGATCTTGCAGCACGACCAGCCGGTGCAGATCACCAT
      CAAGCTGCATGTCACCGAGGACCAGTACACCAATTGGAATACGATTCTGAACCCAGTCAACAACTTGTTGTATGTCGCTC
      TACCGAA
      
      3' Sequence
      Total bases
      457
      NCBI
      3' sequence dataSequence Downloader
      GACATTTTTTAGTTTTCTACTTTTTTTTACGTTTTCGTTCGATTTTTGTTGTTGTTGGATATAGTTTAATATAGTAATTT
      GCGATCAGGCTCTTAACGTGGCTGGCCATAATTGAAAGTCTACCAAAAATGAAGCCGCTCCGAAGACAATTTTCAAAACG
      ATTTTCGACTACATAAACAACGCGAAGATTCAAAAAGGATTGATTTGCTGATTTTCACTGAACACATATTGAGCTCAATA
      TGTCATTGTATCTACGGCTGGCTAATAAAAGTTATTTGCGGAAACTACGAAACAGAATATTGGATTGCTACTGATATTAC
      TTCTTCTTTGTTTCGTTTCTGTGGCGAAAATCGTCCCCTTGCCGACGAACTACTCCTCGATGCTGTAGAGGAAGTAGTAG
      TTCTCGTTGTCGTTAATGGAAGCCGGCGGTGCCTGCGGTGCCTTCCTGGACAGCGGC
      
      Library Information (1)
      Library: HL_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones HL07801 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      Reported As
      Symbol Synonyms
      HL_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)