FB2019_05, released Oct 15, 2019

Clone: Dmel\LD20614

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General Information
Symbol
Dmel\LD20614
Species
D. melanogaster
Name
LD20614
FlyBase ID
FBcl0157193
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG5541
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    LD_pBS_cDNA
    • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pBS vector.
    Strain
      Vector
      pBluescript_SK(-)
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      491
      NCBI
      AA540796
      5' sequence dataSequence Downloader
      ACCGACCGCTCGCGTGTTTTTAGTTTTAGCAGCTAATATAAATACATAAATGCGACTATTTACTTGGGCAATTTGCGACT
AAGATAAGAAACACCTTGAAAGATAAGGCTGGAGCGAAACGGTCTATAATCGGCAGTTCTACGGAACGCTCACGGCGAAG
AGCAGTGCACAGCGGAGGANNCGGTAAACACAACTAGAAAATGAACAAAGAGTGGACCGGAGAATAGAGAAGGCGAAACG
AGCGAGGGCGCGCAAAAGGCACCAGTTAATATGAGTGAATAATTGGCCACAGGCGGATCAGTTGACCAATCATGCGGAAT
ATCAGAAATCTCAAGGATCCGACGACATGGACGCGGATTCTCCTGCCGTTCCTCTCCTGCCTACTCCTCGCTGTCCAGGC
GGATCTGGTGAATGTCACCTGCGGGGAGTATGGCAATACCATCACTTGCGACTGCAATAACTCCGACCAGACCATGGCTT
TGCACTCGTTG
      Library Information (1)
      Library: LD_pBS_cDNA
      Description
      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
      (Rubin et al., 2000)
      Sample preparation
      Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
      Protocol
      RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      LD
      (Rubin et al., 2000)
      LD_pBS
      (Rubin et al., 2000)
      LD_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)