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General Information
Symbol
Dmel\LD10351
Species
D. melanogaster
Name
LD10351
FlyBase ID
FBcl0157255
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI18012
Library
LD_pBS_cDNA
  • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pBS vector.
Strain
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    1,989
    NCBI
    Full Length sequence dataSequence Downloader
    CAGCAGACGGAGCCGTATCGCCGCGATCCGATCGTTAGCCCAAAGATCGCGCTCTTTTATTTCGAATTTTGTGGCTCGCT
    TCACGGGCAATATAGCCATTTATTTCGGTTGTTATCGCTTGTTCGCCTCGATTTTTAGTCGGTAGTTTAACCAAAAATTT
    TATTTTAGACGAGCCGGGCCGGGCAGCTCATAAAAACCAAACATATCGTTCATAAACAATTAGACTGGGGCCAGAGCCAG
    TGCCAGCCAACCAGCCAACCAGCTAGCCAGTCAGCCAGTCAGCCAGCCAGCCAGAGATCTCCGTTGCATCGGTGCAGCTT
    TAATGGAACGTGCGCTGTTCCATGTGCGTGTGGATTGCCCCTTGCCAACGTCCAGAGAGCCATAGGAGTGGAGCGTGATC
    CGGAAACAGCGACGACACAATCAGGATGAAGCGCGACGTGTCCACGTCGACCATCGGCCGGGATGAGGCCCGACGTCCGC
    TGATGGAGGCCTACATGTTCCAGCGGCGTGTGCTGCTCGGCTGCAGTCTGCTCATGGTGGTTTCGCTGCTAATCTGGATA
    GTGGCCATCTCGACCGATCACTGGATCATCATATCCGGCGGAAAGGGCATTTTTATACCCGAATCTCGACGCTTCTTCAT
    GTCCTCGCACTCGGGATTGTGGCGCCATTGCCGGAACACGATAGTGCCCAATGCCATGAGCAATGCCCAGGTGGTGCGCA
    ACTTTAGCTCCATGTCGTACACATCGCAGACGAACATCAACGAGGCGAAGCGAAATCTCTCGCAAATGGACTTCATCAAG
    GAGTTCGCGCACGAGAAGCTGGAAACGTCGGACAACTTTACGGAATCGGCGCGTCGCCACATGTTTGCCCATTGGGTACG
    GGGCGAGGATATGGAGTTTCAGACGCTGCGCCATGCCTTTCGCACCCTGGTGATGAACACCGAGGAGAATCAGCGGCAGT
    TTAATGCCACGGCCATTAAGCCCATACCCATCAATCCGCTGGATGTGCAGGGGAATCATTGAGAGAAATACTTTTGGTTC
    GGCGCTGCAGCGAGTTAAGTACAACAACACTTGGTCCTACTACGTGATCCCGGAGGTGGCGCAGTTAGCCATCTTCAGGA
    ACTGGACGGATTACCCCCTGGTGGTACGACTGCTGGGCACCTACATCCGGGATATCAGCATCCCGGCCTACGTGCTTAAC
    GATGAACGGGTGATACTTATCCTAGTGCCACCGCTGCCGCCCAAGAAGGGTCAGCCCGCCTACTACAGCTATATACCGAA
    CCAGCGCTGTAAGTACATCGACATGTTCCCCAACTCCAATGCTCTGCGAAATGAGCCCGGTTTCGATGACGAACTATTGG
    TCGCTTGGTACTCCCTTTCAGACTATATCCGGACGCAGGCGTCCTTTGCCTGCATAACACTGTTCGTGATGAGCTTGGGT
    GCCGTCTTCTCCTTCTACACGTTCATGAATCCGCGCTACATGTTCAAGCGTCTGGCGGGTGGCATCCACTTGGTGGCTGC
    CTCCACGGCGCTGGTGGTTCTCCAGGTGCTCTTCTCCTCGATTGACTACACCAAAGAGCATCTGTTCTATGCGTATCCTG
    AAGGGGCGCAACTAACTTATGGCTACGGCGTTTATTTGGCCTGGTTCACGTTCGTGGACAATATCCTGTGCGGTGTTATG
    TTCCTCTGGTATTCTGGCAAGAAGAAGGGTGCCAAGGCGCCCAACGACGAGGTGGCCATGGCCGACGAGCCGACCATCAT
    GGGCAGATAACTGGTAACCGGATATCTTTTTGATGGTATTACCTAATGCCCATACTGCGACCAAAGACCAAAGCAATTGG
    CTAACTATCCGCCATTGCTTATCATTTCACTTTTGTTATTTGTTGTTTTCATAATGGCTGTATTTAAAATGTTCGCTTTA
    TAACTTTAAGAATCTCAATGCAATAAAGGAATTGTTTTCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAA
    
    Library Information (1)
    Library: LD_pBS_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

    Sample preparation

    Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

    Comment
    Reported As
    Symbol Synonyms
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Crossreferences
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)