Comment: 0-22 hr AEL
TGAATGCACGTGTTTTCGCTTAACTCACCTGGGCGAATCCCAAAGACCAAAGCAAACCATTAAACTCACATAAAACAATA CAAATTATAAAAACGAAAACACTGTGTTGTGCATCAATCAATATAGTAATATATTGAATTACATCCATACAAAACAGAAA CAAAATTTTTTTTGAGTAATATTTTTTACAAGTGCGCTGAATAACACAAATAAATCAAGAACCCAACAAGTGTTTGAAAA CCCAAAAAATAAATTGGGAAAAACAAAAATTTCTCATAACCGAATATTTTTTTTTTTTGAAAACTTGGGGGAAAAATCAA CACTTTTTTCGACAAGTCACCGAAAACTTAAAAAAAAATTGATTTTTCAAAAAAAGAACCAAGACTCGACACTGCCGAAA AGGCAATATTTTTCAAGTACATTTTTTCGAAGTGTTTTGACAAAACAAAGCAAAATTCTCGAACCAAAGAACAAAATCAC GAAAGGACACCCCAAAACAATCCCTCAATTATAATTGACTTGACTTTGACTTGCAAAGCCGAAGAACTTTCAAAAGACAT GAAGAGGGCAAAACAGAAGTTAACTTGAGCTCGGCTCATGACCAAATCAAAACTGAAATCAAGCAATTTTTTTTTTTTCA ACACAGTGGACGAGCGGCGAACAAACATACACACACAATTGGATTAGTGTTTTGTTTATTTCGGACCCACTGTAACTGTC CCTCGTCCACCTGAAAATACGAAGAAATTCGTCGCCTCCTTGAATTCGTCGCGTGTCGCCGTCGCGTTTCGTTAAATCGC TTATCGTTCATCGCAAATCAACCGATAATCCATCATGCCGAAGATTTTCCTGATCAAAAATCGCCTGCATCAGCAGCAAC AGCGCTTGCTTGAATCGCAAAACTTGCTGCAGCACAAGAATCAGGATGATGAGCGCTTGGTGCCGCCCCTCAGTCCAAGT GGGAGTGGTAGCGGCCCCTCGCCAACGCCCACATCCCAACCACCACCGGAACCGCAAGGTCAGGGGCAACAGGTGCTCGG ACAGGTGCCCGATTCAGATCAACAGCCACTGAGCCTCACGCGCAAACGATTCCACCATCGTCGTCACTACTTCGGACAAT CGCGCCACAGTCTCGATCATCTCAATCAAAATCAGAGCCCGAATCCAAATGCAAATGCAAATCCCAATCAAATACAAAAT CCTGCGGAATTGGAAGTCGAATGCGCCACTGGCCAAGTTCAAAATGAAAACTTTGCCGCCGAGTTGCTGCAGCGTTTGAC ACCAAACACTGCCACCACTGCACAAAATAATATTGTTAACAATTTGGTTAATAATAGCAGAGCCGCCACCTCCGTTTTAG CCACTAAAGACTGTACAATTGAAAATTCCCCAATTAGCATACCAAAAAATCAGCGAGCAGAGGACGAGGAGGAGCAAGAA GACCAGGAAAAAGAGAAGCCCGCAGAGCGGGAGAGAGAGAAGTCCGATGAGAGGACAGAACAGGTAGAAAAGGAGGAGCG GGTGGAGCGCGAAGAGGAGGAGGACGACGAGGTGGATGTCGGCGTAGAGGCGCCACGCCCACGATTCTATAATACCGGCG TGGTCTTAACCCAGGCCCAACGCAAGGAGTATCCCCAAGAGCCCAAGGACTTATCCCTAACCATCGCCAAATCCTCGCCA GCTTCACCGCATATCCACAGTGACTCCGAATCCGATTCGGACTCTGATGGGGGCTGCAAGCTAATTGTGGACGAGAAGCC ACCATTGCCGGTGATCAAACCATTGTCCCTGCGCTTGCGCAGCACACCGCCACCGGCGGATCAACGACCAAGTCCGCCGC CTCCGCGAGATCCCGCGCCCGCTGTCCGCTGCTCGGTGATCCAACGGGCGCCGCAATCCCAATTGCCCACCAGCCGGGCT GGCTTCCTATTGCCCCCCTTGGATCAGCTAGGACCCGAGCAGCAGGAGCCCATCGATTATCACGTGCCGAAGCGAAGGAG CCCCTCTTATGACTCCGATGAGGAGCTGAACGCCAGGCGCCTGGAACGAGCGCGGCAGGTGCGCGAGGCAAGACGACGCA GCACCATTTTGGCCGCACGAGTTCTGCTCGCCCAGTCCCAAAGGCTTAATCCGCGCTTGGTGCGATCCTTGCCCGGTATT TTGGCTGCGGCAGCCGGACACGGACGGAATAGCAGCAGTTCCAGCGGTGCCGCCGGGCAGGGCTTTCAATCGTCCGGTTT TGGCAGCCAAAACAGCGGCAGCGGCTCGTCCAGTGGCAATCAGAACGCCGGCAGCGGCGCTGGTTCACCTGGATCTGGTG CCGGTGGCGGTGGTGGTATGGGCGGTGGTCGCGACGGCCGAGGGAACTACGGACCCAATTCACCGCCAACGGGAGCATTG CCTCCGTTTTATGAGAGCCTCAAGAGCGGCCAACAGAGCACGGCCAGCAACAATACGGGCCAAAGTCCCGGCGCTAATCA TTCGCATTTCAACGCAAATCCAGCGAATTTTCTGCAAAACGCAGCAGCGGCTGCGTACATCATGTCGGCAGGTTCCGGTG GAGGAGGCTGCACCGGAAACGGAGGTGGTGGAGCATCGGGGCCAGGAGGTGGCCCATCGGCAAATAGTGGTGGTGGTGGT GGTGGTGGCGGCGGCAATGGGTACATCAACTGTGGTGGTGTTGGTGGTCCAAACAATAGTCTCGACGGTAATAATCTATT GAACTTCGCCAGTGTTTCTAACTATAACGAATCCAATTCCAAATTCCATAATCATCATCATCATCATCAACACAACAACA ACAACAACAACAATGGTGGTCAAACATCTATGATGGGTCATCCCTTTTACGGCGGTAATCCCTCAGCCTACGGCATAATA CTCAAGGATGAACCGGACATTGAGTACGACGAGGCCAAGATCGATATTGGCACCTTTGCGCAGAACATTATCCAGGCAAC GATGGGCAGCTCCGGTCAGTTCAATGCCAGCGCCTATGAGGATGCTATAATGTCGGACCTGGCCAGTTCGGGTCAGTGTC CCAATGGAGCCGTCGATCCGCTTCAGTTCACAGCCACTCTGATGCTGAGTTCGCAGACCGATCATTTACTGGAGCAGCTG TCCGATGCCGTGGACTTGAGTTCATTCCTGCAAAGGAGCTGCGTGGACGACGAGGAGTCCACCAGTCCGCGGCAGGATTT CGAGCTGGTGTCCACGCCCTCGCTAACGCCGGATTCAGTGACGCCGGTGGAGCAGCACAATACCAATACAACCCAGCTGG ATGTCCTGCACGAGAATCTGTTGACGCAGCTGACCCACAATATAGTCCGGGGCGGCAGCAATCAGCAGCAGCAGCACCAT CAACAGCATGGTGTGCAGCAGCAACAGCAGCAGCAGCATAGTGTGCAGCAGCAACAGCAGCACAATGTGCAACAGCAGCA TGGCGTGCAGCAGCAACATGTCCAGCAGCAACCACCGCCCTCGTATCAGCATGCCACGCGTGGCCTGATGATGCAACAGC AGCCGCAGCACGGCGGCTATCAGCAGCAAGCTGCCATCATGTCGCAACAGCAGCAGCAATTGCTCAGCCAGCAGCAGCAG CAGTCGCACCACCAGCAGCAGCAGCAGCAGCAACATGCCGCTGCCTACCAGCAGCACAACATCTATGCACAGCAGCAACA GCAGCAGCAGCAGCAGCATCATCAACAGCAGCAACAGCAGCAACATCATCACTTCCACCACCAGCAACAACAGCAGCCAC AGCCACAGTCGCATCATTCGCACCATCATGGCCATGGTCATGACAACAGCAACATGTCGCTTCCCTCGCCAACTGCGGCT GCAGCGGCGGCGGCCGCTGCTGCTGCTGCTGCGGCAGCCGCAGCCGCTCATCTGCAGCGTCCGATGAGCAGCAGCAGCAG CTCCGGCGGCACCAATAGCAGCAACAGTAGCGGTGGCAGCAGCAACAGTCCGCTCCTAGATGCAAACGCTGCTGCGGCAG CTGCAGCTGCTTTGTTGGACACAAAGCCACTCATCCAAAGCTTGGGCTTGCCGCCGGATCTGCAGCTTGAGTTTGTGAAC GGCGGCCATGGCATTAAGAACCCGCTGGCCGTGGAGAATGCCCACGGTGGCCATCACCGAATTCGCAACATCGATTGCAT TGATGATCTCAGCAAGCATGGCCACCACTCGCAGCACCAACAGCAGCAGGGCTCGCCGCAGCAGCAAAATATGCAACAAT CGGTGCAACAGCAGTCGGTGCAACAGCAGCAATCCCTGCAGCAGCAACAGCAGCAGCAGCACCATCAGCACCACAGTAAC AGCAGTGCAAGCAGCAATGCCAGCAGTCATGGATCCGCCGAAGCCCTTTGCATGGGCTCATCCGGCGGAGCCAATGAGGA TTCGTCGTCGGGCAACAATAAGTTCGTCTGCCGCGTCTGCATGAAGACATTCTCGCTGCAGCGCCTGCTCAATCGGCACA TGAAGTGTCACTCGGACATCAAGCGGTATCTGTGCACGTTCTGCGGCAAGGGATTCAACGACACCTTCGACCTCAAGCGC CACACGCGCACCCACACGGGCGTCCGGCCGTACAAGTGCAATCTCTGCGAGAAGAGCTTCACGCAGCGCTGTTCCCTCGA GTCGCACTGCCAGAAGGTGCATAGCGTTCAGCACCAGTATGCCTACAAGGAGCGCAGAGCCAAGATGTACGTGTGCGAGG AGTGCGGACACACCACCTGCGAGCCGGAGGTCCACTATCTGCACCTGAAGAACAACCATCCGTTCTCGCCGGCGCTGCTT AAGTTCTACGACAAGCGGCACTTTAAGTTCACCAACTCGCAGTTCGCCAACAATCTGCTCGGCCAGCTGCCCATGCCAGT CCACAATTAGTTGATGGATTTTAGTTATATAGAATAGGAACCATTCGATCGATTCGAATCATTCGAATAACTCGAATCAC TCGAATCACTCGAATCACTTAAGCAAACCAACATTGTGACTTTGAAGTTTTTTGAAAACTGCCCCCCCCTCCTTTTGCGG AGGATACGGAATTATTGGAGATTATGCTTATTATGTATATTTTTGCGTAGTCCTAAGAAAATTAGTTACTAGTTACTAGC TGAATTAGTACCCACTGTATCATGCTACCCCACTTCACTTTGCTGTGCAAATCTCTCTCACTTGAGAAAACTCGAGTTCC CTTTTCTTTATTGTTAATCTTGAATTTTCGTTTATTTCAAGTATGGATCTTTCGAAAAAAAAAACTCGTTGTTAGTCAAA AAGAAAGGTATCGTTAGGACGTAAGAAATGTTTGAAAATGCTTTGAGCAACACTCACACAAATTATGAATGATCGCGTAT ACATATATTAGAAGAAAATATATATTAAATATTTAGCTTTAAGATGTTAAGAGCTACCAACCGAACAAGCTGCAAACGTT GGCATTTATTTGTCTTCAACAAAACAGAACACAAATCAAAAAACAAAAACTTCGAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
